Cyanidin 3 o rutinoside

Flavourzyme^® 1000 L (EC 3.4.11.1, 500 leucine amino peptidase units (LAPU)/g from Aspergillus oryzae) was kindly provided by Novozymes (Bagsværd, Denmark). ACE (EC 3.4.15.1), 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH), picrylsulfonic acid and acetonitrile for HPLC were purchased from Sigma-Aldrich (Madrid, Spain). Captopril (PubChem CID: 44093) and resveratrol were provided by Santa Cruz Biotechnology (Dallas, TX, USA) and Carl Roth (Karlsruhe, Germany), respectively. Folin–Ciocalteu reagent, coumaric acid, quercetin, gallic acid, catechin, and epicatechin were purchased from Fluka/Sigma-Aldrich. 4-Hydroxybenzoic acid, caffeic acid, procyanidin dimer B2, vanillic acid, and malvidin-3-O-glucoside were purchased from Extrasynthése (Lyon, France). Ferulic acid, cyanidin-3-O-rutinoside, and peonidin-3-O-rutinoside were purchased from PhytoLab (Vestenbergsgreuth, Germany). Analytical grade reagents were always used.

Folin–Ciocalteu and p-dimethylaminocinnamaldehyde (DMACA) reagents were acquired from Fluka/Sigma-Aldrich (Madrid, Spain). Gallic acid, quercetin, (+)-catechin, Trolox, fluorescein, 2-thiobarbituric acid, trichloroacetic acid, reduced glutathione (GSH), monochlorobimane, glutathione S-transferase, and 2′,7′-dichlorofluorescein diacetate were purchased from Fluka/Sigma-Aldrich (Madrid, Spain). Cyanidin-3-O-rutinoside was purchased from PhytoLab (Vestenbergsgreuth, Germany). 2,2′-Azobis(2-methylpropionamidine) dihydrochloride (AAPH) was purchased from Acros Organics (Geel, Belgium).
The standard compounds to make up the calibration curve for the quantification of sweet cherry phenolics were prepared in acetone/water/acetic acid (70/29.5/0.5; v/v/v). Every three months, they were prepared again and stored protected from light at −20 °C.

N-hippuryl-His-Leu (Hip-His-Leu) (PubChem CID: 94418), ACE (peptidyl-dipeptidase A, E.C. 3.4.15.1) (PubChem CID: 329770629), glutathione-S transferase from horse liver (PubChem CID: 114886), monochlorobimane (PubChem CID: 114886), 2′,7′-dichlorofluorescein diacetate (PubChem CID: 24894058), 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH, PubChem CID: 57654141), N-1-naftiletilendiamine (PubChem CID: 329754555), HPLC grade acetonitrile and trifluoroacetic acid were provided by Sigma Aldrich (Madrid, Spain). Heparin (PubChem CID: 772) was provided by DeltaLab (Barcelona, Spain). Quercetin, gallic acid, (+)-catechin, p-coumaric acid and (−)-epicatechin were purchased from Fluka/Sigma-Aldrich; caffeic acid, malvidin-3-O-glucoside, vanillic acid, procyanidin dimer B2 and 4-hydroxybenzoic acid were purchased from Extrasynthése (Lyon, France); resveratrol was purchased from Carl Roth (Karlsruhe, Germany); cyanidin-3-O-rutinoside, ferulic acid and peonidin-3-O-rutinoside were purchased from PhytoLab (Vestenbergsgreuth, Germany). The rest of chemical solvents used in this study were of analytical grade.