Anti phospho chk1 ser296 antibody

Manufactured by Cell Signaling Technology

The Anti-phospho-Chk1 (Ser296) antibody is a laboratory tool used to detect the phosphorylation of the Chk1 protein at the serine 296 residue. This antibody can be used in various experimental techniques, such as western blotting, to study the activation and regulation of the Chk1 protein, which plays a crucial role in cell cycle checkpoint control and DNA damage response pathways.

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Anti-CHK1 (55 citations) Phospho-Chk1 (39 citations) Anti-phospho-Chk1 (10 citations) Phospho-CHK1 Ser296 (6 citations) Anti-Chk1 antibody (5 citations)

2 protocols using anti phospho chk1 ser296 antibody

Immunoblotting of DNA Damage Signaling

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Cells were lyzed with NETN lysis buffer (150 mM NaCl, 1 mM EDTA, 10 mM Tris-HCl, pH 8.0, 0.5% NP-40, 1 mM Na₃VO₄, 10 mM NaF, and protease inhibitor cocktail), followed by sonication for 15 seconds. After centrifugation, supernatant was resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose membrane. The following antibodies were used: anti-ZMYND8 antibody (A302–089A, Bethyl Laboratories), anti-cGAS antibody (31659S, Cell Signaling Technology), anti-STING antibody (13647S, Cell Signaling Technology), anti-phospho-TBK1 antibody (5483S, Cell Signaling Technology), anti-TBK1 antibody (3013S, Cell Signaling Technology), anti-phospho-IRF3 (Ser396) antibody (29047S, Cell Signaling Technology), anti-IRF3 antibody (4302S, Cell Signaling Technology), anti-phospho-p65 antibody (3033S, Cell Signaling Technology), anti-p65 antibody (8242S, Cell Signaling Technology), anti-phospho-Chk1 (Ser296) antibody (2349S, Cell Signaling Technology), anti-Chk1 antibody (sc-8408, Santa Cruz Biotechnology), anti-γH2A.X antibody (05–636, Sigma), anti-actin antibody (A2066, Sigma), and anti-H2A.X antibody (10856–1-AP, Proteintech). Proteins were visualized by chemiluminescence with ECL prime (GE Healthcare).

Wang Y., Luo M., Chen Y., Wang Y., Zhang B., Ren Z., Bao L., Wang Y., Wang J.E., Fu Y.X., Luo W, & Wang Y. (2020). ZMYND8 expression in breast cancer cells blocks T-lymphocyte surveillance to promote tumor growth. Cancer research, 81(1), 174-186.

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Protein Expression Analysis by Western Blot

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Anti-Caspase-3 antibody (#9662), anti-PARP antibody (#9542), anti-phospho-Chk1 (Ser296) antibody (#2349), anti-phospho-Chk1 (Ser317) antibody (#2344), anti-phospho-Chk1 (Ser345) antibody (#2348), anti-Chk1 antibody (#2345), anti-phospho-Chk2 (Thr68) antibody (#2661), anti-phospho-Chk2 (Ser516) antibody (#2669), anti-Chk2 antibody (#2662), anti-phospho-p53 (Ser15) antibody (#9284), and anti-phospho-p53 (Ser20) antibody (#9287) were obtained from Cell Signaling Technology; anti-p53 antibody (DO-1; sc-126), anti-PRODH antibody (sc-376401), and HRP-conjugated anti-rat antibody (sc-2032) were from Santa Cruz Biotechnology; anti-α-tubulin antibody (T9026) and anti-γ-tubulin antibody (T6557) were from Sigma Aldrich; anti-p21 antibody (K0081-3) and anti-ATM antibody (PM026) were from Medical and Biological Laboratories; HRP-conjugated anti-rabbit antibody (W4011) and HRP-conjugated anti-mouse antibody (W4021) were from Promega; anti-HA antibody (1867423) was from Roche; anti-DAO antibody (6844-1) was from Abcam.

Nagano T., Nakano M., Nakashima A., Onishi K., Yamao S., Enari M., Kikkawa U, & Kamada S. (2016). Identification of cellular senescence-specific genes by comparative transcriptomics. Scientific Reports, 6, 31758.

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