Buccal samples were collected using Transport Swabs (APCO Laboratory Consumable Plastic, Jordan) or DNA Buccal Swabs (Isohelix, United Kingdom). Participants rinsed their mouths with water and then brushed both sides of their mouth with the collection swab for up to 30 seconds. DNA was extracted from the buccal swabs using either the Qiagen DNA Investigator Kit (Qiagen, USA) or Xtreme DNA Isolation Kit (Isohelix, United Kingdom). DNA extractions were performed according to manufacturer’s recommendations with the exception that the AW2 wash was performed twice for swabs extracted using the Qiagen kit.
Dna investigator kit
Manufactured by Qiagen
Sourced in United States
The Qiagen DNA Investigator Kit is a laboratory product designed for the extraction and purification of DNA from various forensic and other biological samples. It provides a reliable and efficient method for isolating DNA, which is the core function of the kit.
Automatically generated - may contain errors
Lab products found in correlation
7 protocols using dna investigator kit
1
Buccal Swab DNA Extraction Protocol
2
Blinded DNA Sample Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The study was conducted in June of 2014. Twelve blinded genomic DNA samples were kindly provided by George Duncan, Ron Fourney, and Bruce McCord for this study. The samples were obtained under informed consent from volunteers at the Broward Sheriff’s Office. Additionally, the policies and procedures approved by the Institutional Review Board for the University of North Texas Health Science Center in Fort Worth, TX were followed. These single-source samples arrived with arbitrarily assigned Sample Identification Numbers that were used to delineate the samples throughout the study. Extraction and quantification were completed by the sample providers using a Qiagen EZ1Advanced robot with a Qiagen DNA Investigator kit (Qiagen, Valencia, CA, USA) and a Promega Plexor HY real-time kit (Promega, Madison, WI, USA) on a 7500 Real Time instrument (Thermo Fisher Scientific), respectively. The concentrations of these samples ranged from 6.1 nanograms (ng)/microliter (μL) to 40 ng/μL, and a total volume of 7 μL per sample was provided.
3
Buccal DNA Extraction and Genotyping
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Buccal samples were collected using Transport Swabs (APCO Laboratory Consumable Plastic, Jordan) or DNA Buccal Swabs (Isohelix, United Kingdom). After rinsing their mouths with water, participants brushed both sides of their mouth with the collection swab for up to 30 seconds. DNA was extracted from Transport Swabs using the Qiagen DNA Investigator Kit (Qiagen, USA) and from DNA Buccal Swabs using the Xtreme DNA Isolation Kit (Isohelix, United Kingdom). DNA extractions were performed according to manufacturer’s recommendations with the exception that the AW2 wash was performed twice for swabs extracted using the Qiagen kit. DNA samples were genotyped at least two times for each variant and discrepancies were resolved by genotyping a third time. All genotype frequencies were in Hardy-Weinberg equilibrium.
4
Diverse UK Population Genetic Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Buccal swab extracts were chosen from 977 unrelated, adult individuals belonging to five UK-relevant population groups with selfdeclared ancestry: White British, East Asian, South Asian, North-East African and West African.
Individuals gave informed consent for their DNA to be used for research purposes and the ethical approval for this work was granted by the King's BDM research ethics subcommittee HR-18/19-13023. All samples had previously been extracted either using Chelex® 100 Chelating Resin Beads [27] or using the QIAGEN DNA Investigator Kit and the BioRobot EZ1 (QIAGEN) [28] .
Individuals gave informed consent for their DNA to be used for research purposes and the ethical approval for this work was granted by the King's BDM research ethics subcommittee HR-18/19-13023. All samples had previously been extracted either using Chelex® 100 Chelating Resin Beads [27] or using the QIAGEN DNA Investigator Kit and the BioRobot EZ1 (QIAGEN) [28] .
5
High-Throughput Genotyping of Dried Blood Spots
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The dried blood spots on filter paper were sent to the Wellcome Sanger Institute for processing. DNA extraction was carried out using the Qiagen DNA Investigator Kit (No. 56504, Qiagen, Crawley, UK) for high-throughput robotic processing. DNA was eluted in 100 μl TE buffer and stored at − 20 °C for later use. Extracted DNA underwent whole genome amplification (WGA) by primer-extension pre-amplification [37 (link)] or selective WGA [38 (link)] prior to genotyping. Genotyping was performed according to manufacturer’s instruction on the Agena MassARRAY® iPLEX platform (Agena Bioscience, Hamburg, Germany). This system is able to accurately genotype large numbers of samples for multiple SNPs simultaneously. It previously been used in P. falciparum for sequencing validation of novel SNPs [39 (link)]. All genotypes were called from background adjusted peak intensities, which were normalized and called by batch. In brief, batches underwent calling using a heuristic algorithm which identifies intensity ranges for each SNP in single infection samples, and called mixed base loci (“heterozygous”) based on those range thresholds, adjusting for background intensity. Batches were plate based and contained between 96 and 384 samples. These are necessary to generate per assay ranges of intensities to calculate background intensity levels.
6
CCL8 Genotyping in CMV Infection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Patients in cohort 2 (n ¼ 67 D þ /RÀ patients followed for CMV infection after antiviral prophylaxis) were genotyped for a CCL8 SNP. A custom TaqMan SNP qPCR assay for SNP rs3138035 was designed by Life Technologies (assay ID AHAA9YL) based on the CCL8 promoter reference sequence obtained from NCBI. Synthetic oligonucleotides (Integrated DNA Technologies, Coralville, IA) were utilized to validate the qPCR assay. Plasma genomic DNA automated extraction was performed using the DNA Investigator Kit (Qiagen). The qPCR was performed using a StepOne Real-Time PCR instrument and results were analyzed using the StepOne Software v2.2 (Life Technologies, Carlsbad, CA).
7
Buccal DNA Analysis for Forensics
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Buccal swab extracts from 400 unrelated individuals that had previously been analysed using commercially available STR kits and CE were chosen for the study. Individuals gave informed consent for their DNA to be used for research purposes and ethical approval for this work was granted by the King's BDM research ethics subcommittee (HR-16/17-2594). DNA had been previously extracted using either Chelex (Sigma-Aldrich, St Louis, USA) or the DNA Investigator Kit (Qiagen, Heidelberg, Germany).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!