AZ31 (3 wt.% Al, 1 wt.% Zn, 0.3 wt.% Mn) and ZE41 (4 wt.% Zn, 1 wt.% RE, 0.7 wt.% Zr) magnesium alloys were obtained as extruded rods (Magnesium Elektron Company, UK). (3glycidoxypropyl) trimethoxysilane (≥98% GPTMS, Aldrich), titanium IV iso-propoxide (≥97% TIP, Aldrich), acetylacetone, and 2-propanol (≥99.5% ACS reagent, Sigma-Aldrich) were analytical grade. The metallic coupons were cut into discs ( 25 mm x 3 mm thick) and sequentially polished from 180 up to 1000 SiC grit papers. A surface pre-treatment by immersion in 12% HF (puriss p.a., Sigma-Aldrich) was performed to promote the formation of a layer of MgF2 [25] (link). The 12% HF pre-treated alloys were denoted as AZ31 and ZE41. All pre-treated samples were thoroughly washed with ethanol (≥ 96%, Sigma-Aldrich) and dried with air stream.
Gptms
Manufactured by Merck Group
Sourced in United States, Germany
GPTMS is a silane coupling agent used to improve the adhesion between inorganic and organic materials. It acts as a bridge, bonding the two different types of materials together. The core function of GPTMS is to enhance the compatibility and interaction between substrates.
Automatically generated - may contain errors
Lab products found in correlation
Ethanol, by Merck Group (4 mentions)
2 propanol, by Merck Group (2 mentions)
Chitosan, by Merck Group (2 mentions)
Acs reagent, by Merck Group (1 mentions)
Puriss p a, by Merck Group (1 mentions)
Titanium 4 isopropoxide, by Merck Group (1 mentions)
1 propanol, by Thermo Fisher Scientific (1 mentions)
N heptane, by Thermo Fisher Scientific (1 mentions)
Cacodylate buffer, by Merck Group (1 mentions)
Penicillin streptomycin, by Roche (1 mentions)
Ethylenediaminetetraacetic acid edta, by Merck Group (1 mentions)
Porcine gelatin type a, by Merck Group (1 mentions)
Xtt assay kit, by Roche (1 mentions)
Fetal bovine serum (fbs), by Corning (1 mentions)
Glutaraldehyde, by Merck Group (1 mentions)
Ps b p4vp, by Polymer Source (1 mentions)
N n dimethylformamide (dmf), by Merck Group (1 mentions)
N methyl pyrrolidone (nmp), by Merck Group (1 mentions)
Glycidyl methacrylate, by Merck Group (1 mentions)
Ps b p2vp, by Polymer Source (1 mentions)
17 protocols using gptms
1
Magnesium Alloy Surface Pretreatment
2
Silane Grafting on Hydroxypropyl Methylcellulose
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Silane grafting on HPMC involves a Williamson reaction between the hydroxyl function of HPMC and the epoxide group of silane. Using 420 mL of 1-propanol (Acros, Belgium), 1.9l of n-heptane (Acros, Belgium) were stirred. While stirring, 12 g NaOH and 240 g dry HPMC were added to the mixture. The mixture was kept at room temperature for 50 min under nitrogen bubbling. 36 µL of 3-glycidoxypropyltrimethoxysilane (GPTMS) (Aldrich, Germany) (group to be grafted) were added dropwise and temperature was increased to 85 °C. The solution was kept boiling for 3.5 h. Heating was closed and at 40 °C, 30 mL glacial acetic acid was poured to neutralize the reaction. Following 30 min, the mixture was filtered on a buschner. The powder was washed successively four times with 3 L of an acetone/water mixture (85:15 v/v) to eliminate unreacted GPTMS and HPMC-Si powder was dried at 37 °C. The silane percentage commonly used is 0.59 % wt/v [16 (link)].The principle and procedure of the synthesis of Si-HPMC has been described in detail by Bourges et al. [9 (link)].
3
Pectin-based Biomaterial Characterization
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Low methoxy citrus pectin (Classic CU 701, DE 36%, MW 100−400 kDa [36 (link)]) was kindly provided by Herbstreit & Fox (Neuenburg, Germany). Gelatin (porcine Type A, gel strength 300 g bloom), GPTMS, hydrochloric acid (HCl), trypsin, ethylenediaminetetraacetic acid (EDTA), sodium cacodylate buffer, hexamethyldisilane, and ethanol were purchased from Sigma Aldrich (Saint Louis, MI, USA). Dulbecco’s phosphate-buffered saline (DPBS, P0750) without calcium and magnesium (ionic strength 170 mM) was acquired from Biowest (Meda, Italy). Glutaraldehyde and phosphate buffer solution (PBS) were supplied by Merck (Milano, Italy). Dulbecco’s modified Eagle’s medium (H-DMEM) and fetal bovine serum were acquired from Corning Inc (New York, NY, USA). The XTT assay kit and penicillin–streptomycin were acquired from Roche (Basel, Switzerland) and ThermoFisher (Waltham, MA, USA), respectively. Ultrapure water (Arium mini ultrapure lab water system, Sartorius, Varedo, Italy) was used in all experiments. Unless stated otherwise, all chemicals were used as received.
4
Diblock Copolymer Surface Modification
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PS-b-P4VP (number-average molecular weights MnPS = 233 kg/mol and MnP4VP = 133 kg/mol; dispersity Mw/Mn, 1.13; domain spacing, 90.93 nm) and PS-b-P2VP (number-average molecular weights MnPS = 440 kg/mol and MnP2VP = 353 kg/mol; dispersity Mw/Mn, 1.19) diblock copolymers were purchased from Polymer Source and were used without further purification. GPTMS (98%, Sigma-Aldrich) and glycidyl methacrylate (97%, Sigma-Aldrich) were used as surface energy–modifying agents. NMP (99%, Sigma-Aldrich) and DMF (anhydrous, Sigma-Aldrich) were used to dissolve the BCPs, and ethanol (99.9%, Samchun Pure Chemical Co. Ltd.) was used as the nonsolvent.
5
Aptamer-Based Fluorescent Immunoassay for PSA Detection
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Amine-terminated, PSA-specific DNA aptamer
(5′-NH2-(CH2)6-TTTT TAAT TAAA
GCTC GCCA TCAA ATAG CTTT-3′), nonspecific aptamer (5′-H3N-(CH2)6-AAAAATTAATTTCGAGCGGTAGTTTATCGAAA-3′),
ethanolamine (EA), and GPTMS were purchased from Sigma-Aldrich, Germany.
PSA was obtained from Merck Chemicals Ltd. (Beeston, U.K.). For the
electronic assays, PB with pH 7.4 and 10 mM concentration was prepared
from monohydrate phosphate and dinatrium hydrogen phosphate salts.
Anti-PSA antibodies [5A6]-HRP (catalog no. Ab2446) and anti-PSA-fluorescein
isothiocyanate antibodies (catalog no. Ab178776) were purchased from
Abcam, U.K. Luminol (SuperSignal West Femto Substrate Trial Kit 34094)
was purchased from Thermo Scientific, Portugal. All the other reagents
were of analytical grade. Metallic plug adapters for the microfluidic
channel and capillary tubing (BTPE-90) were purchased from Instech
Solomon (PA). One milliliter syringes were purchased from CODAN, Germany.
The liquid flow in the microfluidic channel for the optical immunoassays
was controlled using a NE-300 syringe pump from New Era (NY).
(5′-NH2-(CH2)6-TTTT TAAT TAAA
GCTC GCCA TCAA ATAG CTTT-3′), nonspecific aptamer (5′-H3N-(CH2)6-AAAAATTAATTTCGAGCGGTAGTTTATCGAAA-3′),
ethanolamine (EA), and GPTMS were purchased from Sigma-Aldrich, Germany.
PSA was obtained from Merck Chemicals Ltd. (Beeston, U.K.). For the
electronic assays, PB with pH 7.4 and 10 mM concentration was prepared
from monohydrate phosphate and dinatrium hydrogen phosphate salts.
Anti-PSA antibodies [5A6]-HRP (catalog no. Ab2446) and anti-PSA-fluorescein
isothiocyanate antibodies (catalog no. Ab178776) were purchased from
Abcam, U.K. Luminol (SuperSignal West Femto Substrate Trial Kit 34094)
was purchased from Thermo Scientific, Portugal. All the other reagents
were of analytical grade. Metallic plug adapters for the microfluidic
channel and capillary tubing (BTPE-90) were purchased from Instech
Solomon (PA). One milliliter syringes were purchased from CODAN, Germany.
The liquid flow in the microfluidic channel for the optical immunoassays
was controlled using a NE-300 syringe pump from New Era (NY).
6
PEDOT:PSS Aqueous Dispersion Synthesis
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The aqueous dispersion of PEDOT:PSS (PH1000) was purchased from Heraeus Ltd. with a solid content of 1.0–1.3 wt% and a weight ratio of PEDOT:PSS of 1 : 2.5. PSSA (Mw ∼75 000, 18 wt% in H2O), GPTMS (98%), and dimethyl sulfoxide (DMSO; 98%) were purchased from Sigma-Aldrich Co., Yongin-Si, Gyeonggi-do, Korea.
7
Fabrication of Polymer Membranes for Organic Solvent Nanofiltration
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The Celazole® S26 PBI (MW = 27,000 g mol−1) solution was obtained from PBI Performance Products Inc. (Tuscaloosa, AL, USA). The solution contains 26 wt% PBI and 1.5 wt% lithium chloride (stabilizer) in N,N-dimethylacetamide (DMAc). Non-woven polypropylene fabric (Novatexx 2471) was purchased from Freudenberg Filtration Technologies (Weinheim, Germany). All solvents such as DMAc (TCI, Chuo-ku, Japan), isopropanol (IPA, Daejung, Busan, Korea), acetonitrile (MeCN, Daejung), ethanol (EtOH, Daejung), N,N-dimethylformamide (DMF, Fisher, Al Khobar, Saudi Arabia), N-methyl-2-pyrrolidone (NMP, Alfa, Tewksbury, MA, USA), acetone (Daejung), and tetrahydrofuran (THF, Daejung) were used as received. GPTMS was purchased from Sigma (St. Louis, MO, USA). Rose Bengal (RB; 1017.6 g mol−1), methyl blue (MB; 799.8 g mol−1), and eosin Y (EY; 691.9 g mol−1) were obtained from Daejung, and tetracycline (TC; 444.4 g mol−1) was purchased from TCI. These were used as model solutes in OSN performance testing.
8
Optimized Luminol-Based Chemiluminescence Assay
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Phosphate-buffered saline (PBS) tablets (cat.
no. P4417), 3-(glycidoxypropyl)trimethoxysilane (GPTMS) (440167, 98%
(v/v)), and sodium m-periodate (S1878) were purchased from Sigma-Aldrich.
PBS–0.05% (v/v) Tween (PBST) was prepared by adding 0.5 mL
of Tween-20 solution (cat. no. P7949) to 1 L of PBS buffer. The 5%
(w/v) skim milk (SM) solution was prepared by adding 5 g of SM powder
(70166) to 100 mL of PBST solution. Milli-Q ultrafiltered (UF) H2O (with a resistivity of 18.2 MΩ cm at 25 °C) was
used in the preparation of all the solutions. The luminol–H2O2 substrate solution (ratio 1:1) (cat. no. 1705040,
BioRad) and methyl alcohol (136805) were purchased from Bio-Lab (Israel).
Acetic acid (45731, 99.8% (v/v)) was purchased from Fluka. Hydrochloric
acid (7647010, 37% (v/v)) and hydrogen peroxide solution (7722841,
35%(v/v)) were purchased from Acros Organic.
no. P4417), 3-(glycidoxypropyl)trimethoxysilane (GPTMS) (440167, 98%
(v/v)), and sodium m-periodate (S1878) were purchased from Sigma-Aldrich.
PBS–0.05% (v/v) Tween (PBST) was prepared by adding 0.5 mL
of Tween-20 solution (cat. no. P7949) to 1 L of PBS buffer. The 5%
(w/v) skim milk (SM) solution was prepared by adding 5 g of SM powder
(70166) to 100 mL of PBST solution. Milli-Q ultrafiltered (UF) H2O (with a resistivity of 18.2 MΩ cm at 25 °C) was
used in the preparation of all the solutions. The luminol–H2O2 substrate solution (ratio 1:1) (cat. no. 1705040,
BioRad) and methyl alcohol (136805) were purchased from Bio-Lab (Israel).
Acetic acid (45731, 99.8% (v/v)) was purchased from Fluka. Hydrochloric
acid (7647010, 37% (v/v)) and hydrogen peroxide solution (7722841,
35%(v/v)) were purchased from Acros Organic.
9
Adamantyl-Linked Cyclodextrin Synthesis
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1-Adamantyl bromomethyl ketone, GPTMS, and 1-adamantylamine were purchased from Sigma-Aldrich (USA). α-, β-, and γ-CD were purchased from Tokyo Chemical Industry Co., Ltd. (Japan). 4,4′-Bipyridine was purchased from Alfa Aesar (USA). N,N′-Dimethylformamide (DMF), toluene, and diethyl ether were purchased from Samchun Pure Chemical (South Korea). Sodium hydroxide (NaOH) and methanol were purchased from Daejung Chemicals & Metals Co. Ltd. (South Korea). All reagents were of analytical grade and were used as received without further purification.
10
GO Functionalization for Al 2024 T-3 Adhesion
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A 0.5 wt% aqueous dispersion of graphene oxide (GO) was used as received from Nanografi. APTES, GPTMS, 2-propanol, dicyclohexylcarbodiimide, phenyltrimethoxysilane (Ph(OMe)3Si), HNO3, and Zr(acetylacetonate)4 (Zr(acac)4) were used as received from Sigma-Aldrich. Al 2024 T-3 provided by Turkish Aerospace (TA) was used after cleaning with methylethylketone (MEK), followed by immersion in Bonderite, basic, and acid baths, respectively. Di-water rinse and water-break tests were performed between each of the latter three baths.
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