Fox1nu

All animals were treated according to the European Union rules on animal care. Animal experiments were approved by the Austrian Ministry of Sciences (BMBWF-66.009/0122-V/3b/2019). Approximately 5 × 10⁶ PC-3 cells in 100 µL serum-free medium were subcutaneously injected into flanks of 8-week-old, female, athymic mice (Fox-1^nu, Charles River Laboratories). Xenografts were allowed to grow for 16 days (approx. size: 250 mm³) and mice were intravenously injected via the tail vein with 0.1 µM radiolabeled peptide (10 pmol, ~42 kBq, 100 µL physiological saline) alone (baseline experiments) or co-injected with 2000-fold excess BBN(1–14) (20 nmol; blocking experiments). One hour p.i. of the radiotracers, mice were sacrificed and organs, tumor and blood were removed. Radioactivity in organs and tissues was quantified using a γ-counter (2480 Wizard², PerkinElmer). Organs and tissues were wet-weighted and percentage of injected dose per gram was calculated (% ID/g). Statistical analysis (2-way ANOVA) was performed by GraphPad Prism.

The Institutional Animal Care and Use Committee (IACUC) at the University of Arizona approved all experimental procedures involving animals. Healthy male, weanling nude mice (Fox1nu) were purchased from Charles River Laboratories Inc. After acclimation for a week in the animal facility, mice were injected subcutaneously with a single cell suspension consisting of 10⁷ BEAS-2B cells expressing empty vector, L1 or mutant L1 (five mice for group) in 200 μL of matrigel into each flank. Tumor volume and body weight were recorded after every 2 or 3 days for 65 days.

The Institutional Animal Care and Use Committee (IACUC) at the University of Arizona approved all experimental procedures involving animals. Healthy male, weanling nude mice (Fox1nu) were purchased from Charles River Laboratories Inc. After acclimation for a week in the animal facility, mice were injected subcutaneously with a single cell suspension consisting of 3 X 10⁶ NCI-H520 cells in 200 μL PBS into each flank. When subcutaneous tumors reached a volume of approximately 100 mm³, the mice were randomized into two groups of 6 mice per group. The control group was given PBS and compared to animals given 10 mg/kg/day N6L dissolved in PBS three times per week by intraperitoneal injection. Tumor volume and body weights were recorded every two or three days for 16 days. Tumors were resected following euthanasia and processed for detection of L1-ORF1p expression by immunoblotting.