All tissue sections were processed in Leica TP 1020 Tissue Processor. Samples embedded in paraffin (using HistoCore Arcadia; Leica Biosystems) were cut (5 μm thick) using a microtome (HistoCore AUTOCUT; Leica Biosystems) and mounted on Polysine™ adhesion microscopic slides (Epredia). Hematoxylin and eosin staining was performed according to the protocol described by Suzuki et al.18 Slides were observed and imaged with a light microscope (Motic).
Polysine adhesion microscopic slides
Manufactured by Epredia
Polysine™ adhesion microscopic slides are laboratory-grade slides designed to enhance the adherence of biological samples. These slides feature a specialized coating that promotes the attachment of cells, tissues, or other specimens for microscopic examination. The core function of Polysine™ slides is to provide a secure substrate for sample mounting and analysis.
Automatically generated - may contain errors
2 protocols using polysine adhesion microscopic slides
1
Tissue Processing and Histological Staining
2
Hematoxylin and Eosin Tissue Staining Protocol
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Fixed tissues were processed in Leica TP 1020 Tissue Processor and paraffin
embedded using HistoCore Arcadia (Leica Biosystems). Five micrometers tissue
sections were cut using a microtome (HistoCore AUTOCUT; Leica Biosystems) and
mounted on Polysine™ adhesion microscopic slides (Epredia). Hematoxylin and
Eosin staining was performed following the protocol of Suzuki et al.16 (link) Briefly,
slides containing tissue sections were deparaffinized followed by hydration in a
series of ethanol dilutions. Harris hematoxylin solution (HHS32, Sigma Aldrich,
UK) was added to stain the nuclei (purple) followed by washing to remove excess
stain. Eosin Y solution (HT110216, Sigma Aldrich, UK) was then added to stain
the cytoplasm, collagen and connective tissue (pink). After washing and
dehydration, the slides were mounted and imaging done using a light microscope
Motic BA210 series (Motic, Xiamen, China) integrated ColorVu camera.
embedded using HistoCore Arcadia (Leica Biosystems). Five micrometers tissue
sections were cut using a microtome (HistoCore AUTOCUT; Leica Biosystems) and
mounted on Polysine™ adhesion microscopic slides (Epredia). Hematoxylin and
Eosin staining was performed following the protocol of Suzuki et al.16 (link) Briefly,
slides containing tissue sections were deparaffinized followed by hydration in a
series of ethanol dilutions. Harris hematoxylin solution (HHS32, Sigma Aldrich,
UK) was added to stain the nuclei (purple) followed by washing to remove excess
stain. Eosin Y solution (HT110216, Sigma Aldrich, UK) was then added to stain
the cytoplasm, collagen and connective tissue (pink). After washing and
dehydration, the slides were mounted and imaging done using a light microscope
Motic BA210 series (Motic, Xiamen, China) integrated ColorVu camera.
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