Pma 12 photonic multichannel analyzer

Manufactured by Hamamatsu Photonics

Sourced in Japan

The PMA-12 photonic multichannel analyzer is a compact and versatile device designed for the analysis of optical signals. It features 12 independent photosensitive channels that can simultaneously measure the intensity of light across multiple wavelengths or spatial locations. The PMA-12 is capable of high-speed data acquisition and provides accurate measurements of optical power and spectral characteristics.

Automatically generated - may contain errors

Lab products found in correlation

Nicolet is50 ft ir, by Thermo Fisher Scientific (1 mentions) R928 photomultiplier, by Hamamatsu Photonics (1 mentions) Ff02 438 24, by IDEX Corporation (1 mentions) Ff458 di02 25x36, by IDEX Corporation (1 mentions) Mdo3052, by Tektronix (1 mentions) Multiram spectrometer, by Bruker (1 mentions) Jem arm200f, by JEOL (1 mentions) Eclipse 80i, by Nikon (1 mentions)

Spelling variants of this product

PMA-12 (38 citations)

7 protocols using pma 12 photonic multichannel analyzer

Infrared and Luminescence Spectroscopy Techniques

Check if the same lab product or an alternative is used in the 5 most similar protocols

The Nicolet
iS50 FT-IR from Thermo Scientific was used to collect infrared spectra
at 300 K of samples processed in KBr pellets. The room-temperature
emission spectra utilized excitation at 397, 340, and 300 nm. These
spectra were collected using a FLS1000 photoluminescence spectrometer
from Edinburgh Instruments. The same apparatus was used to collect
the excitation spectra. The ⁵D₀ → ⁷F₂ transition at 619 nm was monitored at room temperature
for the excitation spectra measurements. Emission spectra were also
recorded in response to the 266 nm excitation of a laser diode (CW)
at room temperature and detected using the Hamamatsu PMA-12 photonic
multichannel analyzer. The emission decay profiles were measured at
300 K using either a Ti:sapphire tunable laser or a Nd:YAG laser,
a Hamamatsu R928 photomultiplier, a Jobin-Yvon THR 1000 spectrophotometer,
and a digital LeCroy WaveSurfer oscilloscope. Excitation and emission
spectra were collected at low temperature (5 K) using a temperature-controlled,
continuous-flow liquid helium cryostat: Oxford Model CF 1204. Low
temperature excitation spectra were measured with a Dongwoo Optron
DM151i monochromator and a 150W ozone free lamp. The low temperature
emission spectra were measured using a Dongwoo Optron DM750 monochromator,
an Electro-Optical System INC PbS photodiode, or a Hamamatsu R928
photomultiplier.

Wujczyk M., Targonska S., Boutinaud P., Reeks J.M., Watras A, & Wiglusz R.J. (2022). Emission Enhancement and Energy Transfers in YV0.5P0.5O4 Nanoparticles Codoped with Eu3+ and Bi3+ Ions. Inorganic Chemistry, 61(31), 12237-12248.

+ Open protocol

+ Expand

Organ Elemental Analysis via Thermal Oxidation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Organs (liver, kidneys,
and spleen) were excised from each euthanized animal, minced into
fine pieces, then placed in a furnace and oxidized for 1 h at 100
°C, followed by 1 h at 200 °C, and then 6 h at 450 °C.
The resulting ash was scraped into tubes containing 10 mL of concentrated
sulfuric acid and nitric acid (9:1 ratio) and heated to 90 °C
in a sand bath for 2 h. After cooling, the samples were carefully
diluted in water and centrifuged at 5000 rpm for 30 min. The resulting
pellets were extensively washed with water, with centrifugation increased
to 14,000 rpm. Each pellet was resuspended in 20 μL of water,
and approximately 10 μL was dried on a glass slide. The fluorescence
emission spectra of the dried spots were collected on a custom-built
confocal spectrometer or on a PMA-12 photonic multichannel analyzer
(Hamamatsu Photonics, Japan) with a 532 nm excitation from an OSG
(Hamamatsu Photonics, Japan).

Suarez-Kelly L.P., Sun S.H., Ren C., Rampersaud I.V., Albertson D., Duggan M.C., Noel T.C., Courtney N., Buteyn N.J., Moritz C., Yu L., Yildiz V.O., Butchar J.P., Tridandapani S., Rampersaud A.A, & Carson WE I.I.I. (2021). Antibody Conjugation of Fluorescent Nanodiamonds for Targeted Innate Immune Cell Activation. ACS Applied Nano Materials, 4(3), 3122-3139.

+ Open protocol

+ Expand

Fluorescence Analysis of Tissue Ash Composition

Check if the same lab product or an alternative is used in the 5 most similar protocols

Organs (liver, kidneys, and spleen) were excised from each euthanized animal, minced into fine pieces, then placed in a furnace and oxidized for 1 h at 100 °C, followed by 1 h at 200 °C, and then 6 h at 450 °C. The resulting ash was scraped into tubes containing 10 mL of concentrated sulfuric acid and nitric acid (9:1 ratio) and heated to 90 °C in a sand bath for 2 h. After cooling, the samples were carefully diluted in water and centrifuged at 5000 rpm for 30 min. The resulting pellets were extensively washed with water, with centrifugation increased to 14,000 rpm. Each pellet was resuspended in 20 μL of water, and approximately 10 μL was dried on a glass slide. The fluorescence emission spectra of the dried spots were collected on a custom-built confocal spectrometer or on a PMA-12 photonic multichannel analyzer (Hamamatsu Photonics, Japan) with a 532 nm excitation from an OSG (Hamamatsu Photonics, Japan).

+ Open protocol

+ Expand

Fluorescence and Luminescence Spectroscopy

Check if the same lab product or an alternative is used in the 5 most similar protocols

For the measurement of fluorescence spectra, HeLa cells expressing CFP alone, YFP alone, or a biosensor were plated onto a 35 mm glass-based dish. Cells were observed with an inverted microscope (IX81; Olympus, Tokyo) equipped with an objective lens (UPLAPO 100×/1.35NA oil objective; Olympus). CFP were excited by an FF02-438/24 (Semrock) excitation filter and an FF458-Di02-25x36 (Semrock) dichroic mirror. YFP were excited by an S492/18X (Chroma) excitation filter and a glass dichroic mirror (Olympus). Fluorescence spectra were recorded at 2 nm intervals by using a PMA-12 photonic multichannel analyzer (Hamamatsu Photonics, Hamamatsu, Japan). For the measurement of luminescent spectra, HeLa cells expressing a biosensor were trypsinized and suspended in M199 (ThermoFisher Scientific) containing 3% FBS and 20 mM HEPES. To the cell suspension, 20 μM coelenterazine-h or 3 μM furimazine was added to record luminescence spectra by PMA-12. The obtained fluorescence and luminescence spectra were used for the estimation of energy transfer efficiencies of the biosensors.

Komatsu N., Terai K., Imanishi A., Kamioka Y., Sumiyama K., Jin T., Okada Y., Nagai T, & Matsuda M. (2018). A platform of BRET-FRET hybrid biosensors for optogenetics, chemical screening, and in vivo imaging. Scientific Reports, 8, 8984.

+ Open protocol

+ Expand

Microwave-Assisted Synthesis and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

All precursors (analytical
grade) were commercially available and were used without further purification.
The synthesis was performed on an Ertec Magnum II microwave reactor
with a standard Teflon vessel. The powder X-ray diffraction (XRD)
patterns were obtained on an X’Pert Pro X-ray diffraction system
equipped with a PIXcel detector, a focusing mirror, and Soller slits
for CuKα radiation (λ = 1.54056 Å). The Raman spectra
were measured using a Bruker MultiRAM spectrometer with 2 cm^–1 resolution. A 1064 nm wavelength YAG:Nd laser was used as an excitation
source. The diffuse reflectance spectra were obtained using a Varian
Cary 5E UV–VIS–NIR spectrometer. The temperature-dependent
emission spectra were obtained with a Hamamatsu PMA-12 photonic multichannel
analyzer combined with a BT-CCD sensor. As an excitation source, a
405 nm laser diode was used. The temperature was controlled by a Linkam
THMS600 stage. For lifetime measurements, a Ti-sapphire laser pumped
with Nd:YAG was used as the excitation source. To record decay profiles,
the digital oscilloscope Tektronix MDO3052 was used. The compositions
of samples were determined with energy-dispersive X-ray spectroscopy
(EDS) measurement using a FEI NOVA NanoSEM 140 scanning electron microscope.

Kabański A., Ptak M, & Stefańska D. (2023). Metal–Organic Framework Optical Thermometer Based on Cr3+ Ion Luminescence. ACS Applied Materials & Interfaces, 15(5), 7074-7082.

+ Open protocol

+ Expand

Characterization of Perovskite Nanocrystals

Check if the same lab product or an alternative is used in the 5 most similar protocols

The PL spectra and PLQY of perovskite NCs
were obtained under an
epifluorescence microscope (ECLIPSE 80i, Nikon, Japan) with a 405
nm diode laser (100 mW (CW), Spectra-Physics, USA) and PMA-12 photonic
multichannel analyzer (Hamamatsu, Japan). XRD spectra were obtained
using a Bruker D8 Advance diffractometer with Cu K_α radiation (0.15418 nm). Small-angle measurements were taken with
a knife edge to minimize background noise. Drop-cast samples were
prepared on a silicon substrate. TEM and high-angle annular darkfield
(HAADF)–STEM images were obtained with a JEOL JEM-ARM200F microscope
with an acceleration voltage of 200 kV. Samples were prepared by drop-casting
on a copper grid.

Leng J., Wang T., Tan Z.K., Lee Y.J., Chang C.C, & Tamada K. (2021). Tuning the Emission Wavelength of Lead Halide Perovskite NCs via Size and Shape Control. ACS Omega, 7(1), 565-577.

+ Open protocol

+ Expand

UV-LED Inactivation of Influenza Viruses

Check if the same lab product or an alternative is used in the 5 most similar protocols

Eight different peak wavelength UV-LEDs (Nichia, Tokushima, Japan) and an LP-UV lamp were used to irradiate the viral suspensions in this study (Table 1 and Figure 1). The three individual LEDs were on a printed circuit board (Audio-Q, Shizuoka, Japan, Figure 1a) and connected in series to a current-controlling single power source (PAS40-9, Kikusui Electronics Corp., Kanagawa, Japan). All UV-LEDs controlled the forward current (IF) by the power source for the adjustment of the fluence rate (2.4 mW/cm²). A volume of 0.3 mL virus suspension with an infectivity titer of 1.92 ± 0.13 × 10⁷ (H1N1) or 0.83 ± 0.05 × 10⁷ (H6N2) focus-forming units (FFU)/mL was placed in a stainless steel cylinder cup (10 mm diameter and depth). The UV-LEDs and LP-UV lamp were emitted downward onto the surface of the solution for 2 sec (fluence = 4.8 mJ/cm²). The spectral fluence rates on the surface of samples were measured using an MCPD 3700A multiple wavelength photometer (Otsuka Electronics, Osaka, Japan) and PMA-12 Photonic multichannel analyzer (Hamamatsu photonics, Shizuoka, Japan).

Kojima M., Mawatari K., Emoto T., Nishisaka-Nonaka R., Bui T.K., Shimohata T., Uebanso T., Akutagawa M., Kinouchi Y., Wada T., Okamoto M., Ito H., Tojo K., Daidoji T., Nakaya T, & Takahashi A. (2020). Irradiation by a Combination of Different Peak-Wavelength Ultraviolet-Light Emitting Diodes Enhances the Inactivation of Influenza A Viruses. Microorganisms, 8(7), 1014.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!