Tetramethyl silane

A 10 × 3 × 1 mm piece of nickel, titanium or nitinol with BEAS-2B cells cultured on its surface were fixed for Scanning Electron Microscopy (SEM) in 2% (v/v) glutaraldehyde in 0.1 M cacodylate buffer, and stored until usage at 4 °C. Next, the samples were incubated for 1 h in 1% (v/v) osmium tetroxide (OsO₄) in 0.1 M cacodylate buffer and afterwards the metals were washed three times with Milli-Q ultrapure water. The samples were dehydrated in 30%, 50%, and 70% ethanol, 10 min each, followed by four times dehydration in 100% ethanol for 10, 20, and two times 30 min respectively. Lastly, the samples were dried by incubating for 10 min in 1:1 100% ethanol/tetramethyl silane (Acros, Geel, Belgium) and for 15 min in pure tetramethyl silane followed by air-drying.
After sample preparation, the pieces of metal were placed on a carbon holder and subsequently sputter coated (Leica EM SCD050 and QSG100, Wetzlar, Germany) with 4 nm palladium/gold.
Samples were imaged with a Zeiss Supra 55 SEM (Oberkochen, Germany) using a secondary electron detector at an acceleration tension of 3.0 kilovolt (kV) with 30 µm aperture at 7.2 mm working distance.

Commercial reagents were used without further purification. The ¹H-NMR spectra (500 MHz) were measured using tetramethylsilane (Acros Organics, Fair Lawn, NJ, USA) as internal standard. Thin layer chromatography (TLC) was performed on E. Merck 5554 silica gel plates (Merck KGaA, Darmstadt, Germany). The mass spectra were recorded on a Finnigan LCQ Classic mass spectrometer (Thermo Electron, Langenselbold, Germany).

The 1,2,3-triazole derivatives were synthesized in-house by our research group. All reagents and solvents were obtained from a commercial source (Sinopharm, Beijing, China) and used without further treatment. ¹H NMR and ¹³C NMR spectra were recorded in DMSO-d₆ solution with a Bruker 600 spectrometer (Bruker, Karlsruhe, Germany). Chemical shifts (d) were recorded in parts per million. Tetramethylsilane (Acros, Organics, Brussels, Belgium) was used as an internal reference, and coupling constants were expressed in hertz. High-resolution mass spectra (HRMS) measurements were carried out using a Bruker Micro ToF II mass spectrometer (Bruker, Karlsruhe, Germany).
Hela cell line and DMEM mediumand fetal bovine serum were purchased from ATCC (Manassas, VA, USA) commercially, and Recombinant Human IFN-γ was purchased from R&D systems (Emeryville, CA, USA) commercially. The 3.05 Ntrichloroacetic acid, 4-(dimethylamino) benzaldehyde, and acetic acid were purchased from Sigma Aldrich (St. Louis, MO, USA).