Sas software 7

All statistical analyses were performed using SAS software 7.1 (SAS Institute Inc., Cary, NC, USA); the significance threshold was set at p ≤ 0.05. Data distribution was tested using Shapiro-Wilk test. Statistical analysis of BrdU cell proliferation test, growth curves, and real-time PCR expression analyses of HMGA2 and CD44 were performed using a 2-tailed Mann-Whitney-U test. Times to in vitro wound-scratch closure were analysed by two-tailed t-test. Results from MTS cell proliferation test and flow cytometry were evaluated using Wilcoxon’s two-sample test.

Statistical analysis of data was performed with SAS software 7.1 (SAS Institute Inc., Cary, NC, USA). Means and standard deviations are displayed. For comparison to untreated controls in metabolic activity, cell count and proportions of vital, apoptotic and late apoptotic cells, Dunnett’s test for multiple comparisons was applied. Additionally, Student’s t-tests were performed for comparison between PGE2 concentrations at 1 and 10 μM meloxicam. The confidence levels were set to 95% (p < 0.05; *), 99% (p < 0.01; **) and 99.9% (p < 0.001; ***). All experiments were conducted three times independently, except from cell count and apoptosis rate of doxorubicin and carboplatin response, which were conducted four times and from PGE2 concentrations, which were measured once.

Statistical analysis of data was performed with SAS software 7.1 (SAS Institute Inc., Cary, NC, USA). For comparison of two means, two-tailed t-test was used. The confidence value was set to 5% (p<0.05) and was considered statistically significant.