Two mouse HMGB1 ELISA kits (Uscn Life Science Inc., Wuhan, China; CUSABIO, Houston, TX, USA) were used to measure the levels of HMGB1 in serum, CSF, spinal cord homogenate, and culture medium according to the manufacturers’ protocol. The levels of mouse TNF-α, IL-6, IFN-γ, IL-17A, IL-4, IL-1β, and TGF-β1 in serum, spinal cord homogenate, and culture medium were also measured by ELISA (ELISA kits: TNF-α and IL-6, BioLegend, San Diego, CA, USA; IFN-γ and IL-17A, San Diego, CA, USA; IL-4, IL-1β, and TGF-β1, R&D Systems, Minneapolis, MN, USA). Cytokine concentrations were determined using the relevant standard curves.
Elisa kits tnf α and il 6
Manufactured by BioLegend
Sourced in United States
ELISA kits: TNF-α and IL-6 are laboratory products that measure the concentrations of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) in biological samples using the enzyme-linked immunosorbent assay (ELISA) technique.
Automatically generated - may contain errors
Lab products found in correlation
Il 1β, by R&D Systems (1 mentions)
Tgf β1, by R&D Systems (1 mentions)
Interleukin 4 (il 4), by R&D Systems (1 mentions)
Horse serum, by Thermo Fisher Scientific (1 mentions)
Block it fluorescent oligo, by Thermo Fisher Scientific (1 mentions)
Control sirna, by Integrated DNA Technologies (1 mentions)
Lecithin, by Thermo Fisher Scientific (1 mentions)
Tnf α sirna, by Integrated DNA Technologies (1 mentions)
Penicillin streptomycin p s, by Thermo Fisher Scientific (1 mentions)
Lipopolysaccharide lps, by Merck Group (1 mentions)
Cholesterol, by Merck Group (1 mentions)
Topfluor cholesterol, by Avanti Polar Lipids (1 mentions)
J774a 1 mouse macrophages, by American Type Culture Collection (1 mentions)
2 protocols using elisa kits tnf α and il 6
1
Quantifying Mouse Inflammatory Markers
2
Lipid-Mediated siRNA Delivery and Cytokine Modulation
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DOTAP in chloroform and TopFluor® Cholesterol were from Avanti Polar Lipids (Alabaster, AL). Control siRNA and TNF-α siRNA were from Integrated DNA Technology (IDT) (Coralville, IA). Lecithin was from Alfa Aesar (Ward Hill, MA). Cholesterol, lipopolysaccharide (LPS), and tetrahydrofuran (THF, HPLC grade) were from Sigma-Aldrich (St. Louis, MO). The PEG2000-hydrazone-stearic acid conjugate (PHC) was synthesized as previously described (Zhu et al., 2013a (link), Zhu et al., 2013b (link)). FITC-labeled siRNA (BLOCK-iT™ Fluorescent Oligo) was from Invitrogen (Carlsbad, CA). ELISA kits (TNF-α and IL-6) were from BioLegend (San Diego, CA). Ultrafiltration devices (Amicon, 30 KDa molecular weight cutoff (MWCO)) were from Millipore Sigma (Burlington, MA). Dialysis devices (Float-A-Lyzer G2, 50 KDa MWCO) were from Spectrum Laboratories (Waltham, MA). All other solvents were of HPLC grade and were from Fisher Scientific (Waltham, MA). Mouse J774A.1 macrophages and mouse MPRO promyelocytic cell line were from the American Type Culture Collection (Manassas, VA). Dulbecco’s Modified Eagle Medium (DMEM), Iscove’s Modified Dulbecco’s Medium (IMDM), fetal bovine serum, horse serum and penicillin/streptomycin (P/S), all from Gibco (Grand Island, NY).
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