Hmec human mammary epithelial cells and derivatives

MCF-10A cells (ATCC) and derivatives were cultured in Dulbecco’s Modified Eagle Medium (DMEM) Nutrient Mixture F-12 Ham 1:1 powder (Gibco) supplemented with 5% horse serum (Invitrogen), 20 ng/ml epidermal growth factor, 10 μg/ml insulin, 500 μg/ml hydrocortisone, 100 ng/ml cholera toxin, 1% penicillin/streptomycin, and Plasmocin prophylactic. BJ human foreskin fibroblasts, MDA-MB-231, and MDA-MB-436 were cultured in DMEM high glucose supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin. HMEC human mammary epithelial cells and derivatives (Lonza) were cultured in mammary epithelium basal medium MEBM (Lonza) plus MEBM SingleQuot Supplements. Komen Tissue bank cells (KTB-34) were cultured as previously described (67 (link)). HuVEC cells were grown in EGM endothelial cell growth medium Bulletkit (Lonza; CC-3124). Mycoplasma testing was routinely performed using mycoplasma PCR detection kit (LiliF, 102407-870) in all cell lines.

MCF-10A cells (ATCC) and derivatives were cultured in Dulbecco’s Modified Eagle Medium Nutrient Mixture F-12 Ham 1:1 powder (Gibco) supplemented with 5% horse serum (Invitrogen), 20 ng/ml epidermal growth factor (EGF), 10 μg/ml insulin, 500 μg/ml hydrocortisone, 100 ng/ml cholera toxin, 1% penicillin/streptomycin and Plasmocin^® prophylactic. BJ human foreskin fibroblasts, MDA-MB-231 and MDA-MB-436 were cultured in Dulbecco’s Modified Eagle Media high glucose (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. HMEC human mammary epithelial cells and derivatives (Lonza) were cultured in mammary epithelium basal medium MEBM ^™(Lonza) plus MEBM SingleQuot^™ Supplements. Komen Tissue bank cells (KTB-34) were cultured as previously described (66 (link)). Mycoplasma testing was routinely performed using mycoplasma PCR detection kit (LiliF, 102407–870) in all cell lines.