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Human endothelial medium

Manufactured by Thermo Fisher Scientific
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Human Endothelial Medium is a cell culture medium designed to support the growth and maintenance of human endothelial cells. It provides the necessary nutrients, growth factors, and other components required for the in vitro culture of these cell types.

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Lab products found in correlation

Pen strep, by Thermo Fisher Scientific (1 mentions) Mouse egf, by Thermo Fisher Scientific (1 mentions) Human bfgf, by R&D Systems (1 mentions) Mouse pdgf, by Thermo Fisher Scientific (1 mentions) Non adherent 24 well plates, by Corning (1 mentions) Mouse oncostatin m, by R&D Systems (1 mentions) Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions) Dmem f12, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Human endothelial basal growth medium Human endothelial serum-free medium

2 protocols using human endothelial medium

1

Conditioned Media Effects on Endothelial Cells

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For conditioned media (CM), equal amounts of LM and MM cells with or without HMGA2 overexpression were cultured with serum-free media for 48 hours, and then the CM were stored at −70°C until use. HUVEC (kindly provided by Dr. W. Muller, NU) were cultured in Human Endothelial Medium (Gibco) plus 10% FBS.
Li Y., Qiang W., Griffin B.B., Gao T., Chakravarti D., Bulun S., Kim J.J, & Wei J.J. (2020). HMGA2-mediated tumorigenesis through angiogenesis in leiomyoma. Fertility and sterility, 114(5), 1085-1096.
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2

Spheroid Formation from Sorted Cells

Cited 3 times
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Mouse sorted cells at 1 × 103 were transferred to non-adherent 24 well plates (Corning) with spheroid-forming media5 (link), 7 (link), 47 (link) [1:2 ratio of DMEM F12 (Gibco) and Human Endothelial Medium (Gibco) supplemented with 3.75% Chicken Extract (US Biological), 0.1 mM β-ME (Invitrogen), 1% Non-essential amino acids (Gibco), 1% Pen-strep (Gibco), 1% N2 (Gibco), 2% B27 (Gibco), 20 ng/mL human bFGF (R&D Systems), 20 ng/mL mouse PDGF (Peprotech), 20 ng/mL mouse oncostatin M (R&D Systems), 20 ng/mL mouse IGF-1 (Peprotech), 20 ng/mL mouse EGF (Peprotech)]. After 7 days, the spheroid efficiency was determined.
Yang M., Arai A., Udagawa N., Hiraga T., Lijuan Z., Ito S., Komori T., Moriishi T., Matsuo K., Shimoda K., Zahalka A.H., Kobayashi Y., Takahashi N, & Mizoguchi T. (2017). Osteogenic Factor Runx2 Marks a Subset of Leptin Receptor-Positive Cells that Sit Atop the Bone Marrow Stromal Cell Hierarchy. Scientific Reports, 7, 4928.
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