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Anti p62 gtx100685

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The Anti-p62 (GTX100685) is a primary antibody that recognizes the p62/SQSTM1 protein. p62 is a multifunctional adaptor protein involved in various cellular processes. This antibody can be used for the detection of p62 in various applications such as Western blotting and immunohistochemistry.

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Spelling variants of this product

Anti-p62 (11 citations) GTX100685 (6 citations)

2 protocols using anti p62 gtx100685

1

Immunoblotting and Immunohistochemistry Techniques

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The relevant procedures have been described in a previous report [8 (link)]. Proteins were reacted with one of the following: anti-LC3A (#4599), anti-LC3B (#3868), anti-PARP (#5625), anti-GADD153 (#2895), anti-GRP78 (#3177), anti-t-Akt (#9272), anti-p-Akt Ser473 (#9271), anti-p-ERK (#4370), anti-p-p70S6K Thr389 (#9234) purchased from Cell Signaling (Danvers, MA), anti-p62 (GTX100685) obtained from GeneTex (Irvine, CA) or monoclonal anti-β-actin (Sigma, AC-40). Immunohistochemistry (IHC) was carried out with polyclonal anti P-gp, followed by anti-goat IgG (Santa Cruz Biotechnology) conjugated to horseradish peroxidase. Hematoxylin was used for counterstaining.
Chiu L.Y., Hu M.E., Yang T.Y., Hsin I.L., Ko J.L., Tsai K.J, & Sheu G.T. (2015). Immunomodulatory Protein from Ganoderma microsporum Induces Pro-Death Autophagy through Akt-mTOR-p70S6K Pathway Inhibition in Multidrug Resistant Lung Cancer Cells. PLoS ONE, 10(5), e0125774.
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2

Western Blot Analysis for Apoptosis, Autophagy, and Signaling Pathways

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Western blot analysis for apoptosis, autophagy and signaling pathway regulated proteins. The relevant procedures have been previously described.3 (link) Proteins were reacted with one of the followings: anti-LC3B (#3868), anti-cleavaged Poly(ADP-ribose) Polymerase (cPARP) (#5625) purchased from Cell Signaling (Danvers, MA), anti-p62 (GTX100685) obtained from GeneTex (Irvine, CA), or monoclonal anti-β-actin (Sigma, AC-40). Chloroquine (CQ, 10 μM) was added with DOC or VCR or VER in fresh medium for 48 hrs treatment and cells were harvested for analysis.
Wong B.S., Chiu L.Y., Tu D.G., Sheu G.T, & Chan T.T. (2020). Anticancer Effects of Antihypertensive L-Type Calcium Channel Blockers on Chemoresistant Lung Cancer Cells via Autophagy and Apoptosis. Cancer Management and Research, 12, 1913-1927.
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