The miR-21a-5p target genes in the BV-2 cells were determined using the target prediction databases TARGETSCAN-VERT (http://www.targetscan.org ) and MIRDB (http://mirdb.org/miRDB/ ). The results showed that STAT3 is one of the target genes of miR-21a-5p. The plasmids pmirGLO-STAT3 WT-3′-UTR and pmirGLO-STAT3 MUT-3′-UTR were purchased from Gene Pharma (Suzhou, Jiangsu Province, China). BV-2 cells were transfected with the pmirGLO-WT/Mut-STAT3, miR-21a-5p mimics, and miR-21a-5p NC (Gene Pharma), using Lipofectamine 2000 according to the manufacturer’s instructions (Thermo Fisher Scientific). A Dual-Luciferase Reporter Assay System (Promega Corporation, Madison, WI, USA) was used to detect firefly and renilla luciferase activity.
Pmirglo stat3 wt 3 utr
Manufactured by GenePharma
Sourced in China
The PmirGLO-STAT3 WT-3'-UTR is a reporter plasmid that contains the 3' untranslated region (UTR) of the STAT3 gene. It is designed for use in studies related to the post-transcriptional regulation of the STAT3 gene.
Automatically generated - may contain errors
2 protocols using pmirglo stat3 wt 3 utr
1
Validating miR-21a-5p Regulation of STAT3
2
Validating miR-21a-5p Regulation of STAT3
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The miR-21a-5p target genes were acquired using the following programs: TargetScanVert and miRDB.
The prediction results show that STAT3 is one of the target genes of miR-21a-5p. The pmirGLO-STAT3
WT-3′-UTR or pmirGLO-STAT3 MUT-3′-UTR plasmids were purchased from Gene Pharma (Suzhou, China). Subsequently, the pmirGLO-WT/Mut-STAT3, miR-21a-5p mimics, and miR-21a-5p NC were all transfected into BV-2 cells according to the instructions of Lipo2000 (Thermo Fisher Scienti c, PA, USA). Finally, Dual-Luciferase Reporter Assay System (Promage Corporation, USA) was used to detect re y and renilla luciferase activities.
The prediction results show that STAT3 is one of the target genes of miR-21a-5p. The pmirGLO-STAT3
WT-3′-UTR or pmirGLO-STAT3 MUT-3′-UTR plasmids were purchased from Gene Pharma (Suzhou, China). Subsequently, the pmirGLO-WT/Mut-STAT3, miR-21a-5p mimics, and miR-21a-5p NC were all transfected into BV-2 cells according to the instructions of Lipo2000 (Thermo Fisher Scienti c, PA, USA). Finally, Dual-Luciferase Reporter Assay System (Promage Corporation, USA) was used to detect re y and renilla luciferase activities.
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