MCF-7 and CCD-18Co cells obtained from ATCC (Manassas, VA, USA) were grown in Dulbecco’s modified Eagle medium (DMEM). T47D, BT-474, ZR-75-1, and HCC1428 cells obtained from Korean Cell Line Bank (KCLB, Seoul, Korea) were grown in Roswell Park Memorial Institute (RPMI) 1,640 medium supplemented with 10% fetal bovine serum. MCF-10A cells were maintained in DMEM/F12 supplemented with 5% horse serum, epidermal growth factor (EGF), hydrocortisone, cholera toxin, and insulin in 5% CO2 at 37 °C. All the cell lines were tested for mycoplasma contamination and authenticated by STR DNA profiling. Tam-R cells were generated by a 12-month treatment of MCF-7 cells with 4-HT (1 μM).23 (link) Transient transfection using Oligofectamine (Invitrogen, Carlsbad, CA, USA) was performed as described previously.21 (link) Immunoblotting was performed using the antibodies listed in the Supporting Information Table 4 .
Hcc 1428
Manufactured by Korean Cell Line Bank
HCC-1428 is a cell line derived from a human hepatocellular carcinoma. It is a widely used in vitro model for liver cancer research.
Automatically generated - may contain errors
Lab products found in correlation
Bt 474, by Korean Cell Line Bank (2 mentions)
Zr 75 1, by Korean Cell Line Bank (2 mentions)
Oligofectamine, by Thermo Fisher Scientific (1 mentions)
Ccd 18co, by American Type Culture Collection (1 mentions)
Mcf 7, by American Type Culture Collection (1 mentions)
High glucose dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Hs578t, by Korean Cell Line Bank (1 mentions)
Hcc1419, by Korean Cell Line Bank (1 mentions)
Mcf 7, by Korean Cell Line Bank (1 mentions)
3 protocols using hcc 1428
1
Breast Cancer Cell Culture and Transfection
2
Maintenance of Breast Cancer Cell Lines
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MDA-MB-231 cells were kindly provided by Dr. Jeong-Seok Nam (GIST, Gwangju, Korea) and maintained in Dulbecco’s Modified Eagle’s Medium (high glucose; Gibco, Billings, MT, USA) containing 10% fetal bovine serum (FBS), 50 µg/mL streptomycin, and 50 units/mL penicillin. ZR-75-1, T47D, HCC-1428, HCC-1419, and Hs578T were purchased from the Korean Cell Line Bank and maintained as specified in the datasheet.
3
Culturing Breast Cancer Cell Lines
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