Rabbit anti gβ

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Rabbit anti-Gβ is a primary antibody raised in rabbits against the G-protein beta subunit (Gβ). It is used for the detection and analysis of Gβ proteins in various experimental applications.

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Lab products found in correlation

Mouse anti ha agarose, by Merck Group (1 mentions) Rabbit anti flag, by Merck Group (1 mentions) Mouse anti flag, by Merck Group (1 mentions) Mouse anti ha, by Fortrea (1 mentions) Rabbit anti mouse peroxidase, by Merck Group (1 mentions) Goat anti rabbit peroxidase, by Merck Group (1 mentions) Rabbit anti tie2, by Santa Cruz Biotechnology (1 mentions) Goat anti histone h3, by Santa Cruz Biotechnology (1 mentions) Goat anti tie2, by R&D Systems (1 mentions) Mouse anti γ tubulin, by Merck Group (1 mentions) Mouse anti ptyrosine 4g10, by Merck Group (1 mentions) Donkey anti goat fitc, by OriGene (1 mentions) D glucose, by Merck Group (1 mentions)

3 protocols using rabbit anti gβ

Immunoblotting Antibody Protocols

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The
following primary antibodies were used
for immunoblotting (dilutions indicated): rabbit anti-Gβ (Santa
Cruz, catalog no. sc-378, 1:15000), mouse anti-N-methyl-d-aspartate receptor-1 (NMDAR1) (BD Pharmingen, catalog no.
556308, 1:2000), mouse anti-postsynaptic density-95 (PSD-95) (Neuromab,
catalog no. 75-028, 1:20000), mouse anti-glyceraldehyde-3-phosphate
dehydrogenase (GAPDH) (Millipore, catalog no. MAB374, 1:20000), and
mouse anti-syntaxin-1 (Santa Cruz, catalog no. sc-12736, 1:2000).
Horseradish peroxidase-conjugated secondary antibodies were obtained
from Perkin-Elmer and used at the following dilutions: goat anti-rabbit
(1:20000) and goat anti-mouse (1:10000 for NMDAR1 and syntaxin and
1:20000 for PDS-95 and GAPDH).

Betke K.M., Rose K.L., Friedman D.B., Baucum AJ I.I., Hyde K., Schey K.L, & Hamm H.E. (2014). Differential Localization of G Protein βγ Subunits. Biochemistry, 53(14), 2329-2343.

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Antibody-Based Protein Detection

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mouse anti-HA-agarose (Sigma, A2095), mouse anti-FLAG (Sigma, F3165) mouse anti-HA (Covance, 901514, 1:750), rabbit anti-FLAG (Sigma, F7425, 1:100), and rabbit anti-Gβ (Santa Cruz, sc-378, 1:10,000 and 1:5000) were used.

Yim Y.Y., Betke K.M., McDonald W.H., Gilsbach R., Chen Y., Hyde K., Wang Q., Hein L, & Hamm H. (2019). The in vivo specificity of synaptic Gβ and Gγ subunits to the α2a adrenergic receptor at CNS synapses. Scientific Reports, 9, 1718.

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Quantification of Angiopoietin-2 Signaling

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soluble Tie2-Fc chimera human (sTie2) (C-68028, Promokine, Germany, 5 µg/mL), D-Glucose (G7021, Sigma, Germany 5 mM or 30 mM), gelatin from porcine skin (48720, Fluka, Bucharest, Romania, 1% solution in PBS) was used to coat cell culture and assay plates. The antibodies used were: mouse anti-NDPK-B (Kamiya Biomedicals, Seattle, WA, USA; MC-412); mouse anti-Ang2 (sc-74403, Santa Cruz Biotechnology, Dallas, TX, USA); rabbit anti-Tie2 (Santa Cruz Biotechnology, Dallas, TX, USA, sc-324); mouse anti-pTyrosine 4G10 (Millipore, Burlington, MA, USA; 05-321); mouse anti-γ-tubulin (Sigma-Aldrich, St. Louis, MO, USA; T6557); rabbit anti-Gβ (Santa Cruz Biotechnology, Dallas, TX, USA; sc-378); goat anti-Histone H3 (Santa Cruz Biotechnology, Dallas, TX, USA; sc-8654); Isolectin-TRITC (Sigma-Aldrich, St. Louis, MO, USA; L5264); goat anti-Tie2 (R&D Systems, Minneapolis, MN, USA; AF762); mouse anti-Tie2/TEK clone AB33 (Merck, Darmstadt, Germany; 05-584) rabbit anti-goat peroxidase (Sigma-Aldrich, St. Louis, MO, USA; A8919); rabbit anti-mouse peroxidase (Sigma-Aldrich, St. Louis, MO, USA; A9044); goat anti-rabbit peroxidase (Sigma-Aldrich, St. Louis, MO, USA; A9169); donkey anti-goat-FITC (Acris Antibodies, Hiddehausen, Germany; R1254F); swine anti-rabbit-FITC (Dako Agilent Technologies, Santa Clara, CA, USA; F0205)

Chatterjee A., Eshwaran R., Huang H., Zhao D., Schmidt M., Wieland T, & Feng Y. (2020). Role of the Ang2–Tie2 Axis in Vascular Damage Driven by High Glucose or Nucleoside Diphosphate Kinase B Deficiency. International Journal of Molecular Sciences, 21(10), 3713.

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