FACS sorted MSC subsets were first grown for 2 weeks in phenol red-free α-MEM plus 10% fetal bovine serum and 1% penicillin/streptomycin for 2 weeks with one half medium change every 3 days to reach 70% confluency. For adipocyte differentiation, MSC subsets were cultured in adipogenesis medium (MesenCult basal medium + mouse MesenCult adipogenic stimulatory supplement; Cat no. 05503; Stem Cell Technologies) for further 3 weeks by following manufacturer’s instructions. Briefly, medium was changed every 3–4 days and wells were then stained with Oil Red O solution (Cat no. LL-0052; Lifeline Cell Technology, Frederick, MD). For osteoblast differentiation, MSC subsets were cultured in osteogenic medium (MesenCult basal medium + mouse MesenCult osteogenic stimulatory kit; Cat no. 05504; Stem Cell Technologies) for further 3 weeks by following manufacturer’s instructions. Briefly, medium was changed every 3–4 days and wells were then stained with 2% Alizarin Red S (Cat no. CM-0058; Lifeline Cell Technology, Frederick, MD).
Mouse mesencult adipogenic stimulatory supplement
Manufactured by STEMCELL
Mouse MesenCult adipogenic stimulatory supplement is a medium formulation designed to promote the differentiation of mouse mesenchymal stem cells into adipocytes.
Automatically generated - may contain errors
2 protocols using mouse mesencult adipogenic stimulatory supplement
1
Adipogenic and Osteogenic Differentiation of MSC Subsets
2
Adipogenic and Osteogenic Differentiation of MSC Subsets
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FACS sorted MSC subsets were first grown for 2 weeks in phenol red-free α-MEM plus 10% fetal bovine serum and 1% penicillin/streptomycin for 2 weeks with one half medium change every 3 days to reach 70% confluency. For adipocyte differentiation, MSC subsets were cultured in adipogenesis medium (MesenCult basal medium + mouse MesenCult adipogenic stimulatory supplement; Cat no. 05503; Stem Cell Technologies) for further 3 weeks by following manufacturer’s instructions. Briefly, medium was changed every 3–4 days and wells were then stained with Oil Red O solution (Cat no. LL-0052; Lifeline Cell Technology, Frederick, MD). For osteoblast differentiation, MSC subsets were cultured in osteogenic medium (MesenCult basal medium + mouse MesenCult osteogenic stimulatory kit; Cat no. 05504; Stem Cell Technologies) for further 3 weeks by following manufacturer’s instructions. Briefly, medium was changed every 3–4 days and wells were then stained with 2% Alizarin Red S (Cat no. CM-0058; Lifeline Cell Technology, Frederick, MD).
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