T1 sm 100 inverted microscope

Mouse fibroblast L929 cells were cultured in contact with the CM-CTS solution to evaluate its biocompatibility through MTT assay. L929 cells were inoculated into 96-well plates at an initial density of 1.5 × 10⁴/well in a 200 μL culture medium overnight. Subsequently, the original medium was replaced with various levels (1.0, 2.0, and 4.0 mg/mL) of CM-CTS solutions. After 24 and 48 h, the cell morphology was recorded with a T1-SM 100 inverted microscope (Nikon Corporation, Tokyo, Japan). The relative proliferation rate of fibroblasts was examined with the MTT method, as previously described [40 (link),41 (link)].

Hydrogen peroxide (H₂O₂) was used to establish an oxidative damage model in Schwann RSC96 cells. RSC96 cells were inoculated into 96-well plates at a density of 1.5 × 10⁴/well in a 200 μL culture medium overnight. Subsequently, the original medium was replaced with various levels (100, 200, 400, and 800 μg/mL) of CM-CTS solutions for 24 h. Then, the pre-treated cells were damaged with H₂O₂ for 24 h. Finally, the cell morphology was recorded with a T1-SM 100 inverted microscope (Nikon Corporation, Tokyo, Japan). The relative proliferation rate of the Schwann cells was examined with an MTT assay, and Hoechst 33258 fluorescence staining was performed to observe the cell viability of the damaged RSC96 cells.