For the immunofluorescence analysis (IFA), the samples were fixed in 4% paraformaldehyde and then washed three times in PBS. The samples were then permeabilized with 0.5% Triton X-100 for 30 min and then blocked with PBS–bovine serum albumin for 1 h at room temperature. Next, the samples were incubated with the primary antibodies, including anti-NbTMP1 ascites (mouse), or with negative serum and Nbβ-tubulin (rabbit) antibody which was used to label meronts of N. bombycis [20 (link)]. After washing three times with PBS, Alexa 488 conjugate Goat anti-Mouse IgG and Alexa 568 conjugate Goat anti-Rabbit IgG (Thermo Fisher Scientific) were used to detect the bound primary antibodies, and 4′,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific) was used to label the nucleus. The results were observed under a confocal laser scanning microscope (Olympus, Tokyo, Japan).
Alexa 568 conjugate goat anti rabbit igg
Manufactured by Thermo Fisher Scientific
Sourced in United States
Alexa 568 conjugate Goat anti-Rabbit IgG is a secondary antibody conjugated with the Alexa Fluor 568 fluorescent dye. It is designed to recognize and bind to rabbit immunoglobulin G (IgG) antibodies, allowing for the visualization and detection of target proteins or molecules in various biological applications.
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2 protocols using alexa 568 conjugate goat anti rabbit igg
1
Immunofluorescence Analysis of N. bombycis Meronts
2
Immunolabeling of N. bombycis meronts
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The samples were xed with 4% paraformaldehyde and washed three times with PBS. Then the samples were permeabilized with 0.5% Triton X-100 for 30 min and then blocked with PBS-bovine serum albumin for 1 h at room temperature. Next, the samples were incubated with the primary antibodies including anti-NbTMP1 ascites (mouse) and antibody of Nbβ-tubulin (rabbit), which was used to label meronts of N. bombycis (20) . After washing three times with PBS, Alexa 488 conjugate Goat anti-Mouse IgG and Alexa 568 conjugate Goat anti-Rabbit IgG (Thermo Fisher, Santa Clara, CA, USA) were used to detect the bound primary antibodies, and 4', 6-diamidino-2-phenylindole (DAPI) (Thermo Fisher, Santa Clara, CA, USA) was used to label the nucleus. The results were observed by a confocal laser scanning microscope (Olympus, Japan).
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