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Mhcii alexa fluor 700

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MHCII-Alexa Fluor 700 is a fluorescently labeled antibody that specifically binds to major histocompatibility complex class II (MHCII) proteins. Alexa Fluor 700 is the fluorescent dye conjugated to the antibody, allowing for detection and analysis of MHCII-expressing cells using flow cytometry or other fluorescence-based techniques.

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Lab products found in correlation

Tyrosine hydroxylase, by Cell Signaling Technology (2 mentions) Cd11b percp cy5, by Thermo Fisher Scientific (2 mentions) Cd3 bv605, by BioLegend (2 mentions) Nf kb, by Cell Signaling Technology (2 mentions) Pnfkb, by Cell Signaling Technology (2 mentions) Alexa fluor 488 anti rabbit igg, by Thermo Fisher Scientific (2 mentions) Cd11c apc efluor780, by Thermo Fisher Scientific (2 mentions) B220 apc, by Thermo Fisher Scientific (2 mentions) Fixable viability dye aqua cd45 bv711, by BD (2 mentions) F4 80 eflour450 f4 80 pe, by BD (2 mentions) Tubb3 alexafluor488, by BioLegend (2 mentions) Anti rabbit igg apc, by Thermo Fisher Scientific (2 mentions) Caspase 1, by Genentech (2 mentions) F4 80 pe, by Thermo Fisher Scientific (2 mentions) Cd206 pe cy7, by BioLegend (2 mentions)

Spelling variants of this product

MHCII (84 citations) Alexa Fluor 700 (33 citations) Alexa Fluors (21 citations) Alexa Fluor 700-anti-MHCII (3 citations) MHCII-Alexa-700 (2 citations)

2 protocols using mhcii alexa fluor 700

1

Comprehensive Immunophenotyping of Adipose Tissue

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For flow cytometry analysis, the following antibodies were used: Fixable Viability Dye Aqua; CD45-BV711 (30F11: cat#563709) (BDBioscience); F4/80-eFlour450; F4/80-PE (BM8; cat#48-4801-82 & 12-4801-82); CD11b-PerCPCy5.5 (M1/70; cat#45-0112-82); CD11c-APC-eFluor780 (N418; cat#47-0114-82); B220-APC (RA3-6B2; cat#17-0452-82); MHCII-Alexa Fluor 700 (M5/114.15.2; cat#56-5321-82) (eBioscience) and CD3-BV605 (17A2; cat#100237); CD206-PECy7 (C068C2; cat#141709) (Biolegend). For western blot analysis, the following antibodies were used: pHSL (Ser660; cat#4126), pHSL (Ser563; cat#4139), HSL (cat#4107), ATGL (cat#2439), Actin (cat#4967), UCP-1 (cat#14670), pNFkB (cat#3033), NFkB (cat#8242), (Cell Signaling), Caspase-1 (Genentech). For whole mount staining: F4/80-PE (BM8; 12-4801-82; eBioscience), TUBB3-AlexaFluor488 (TUJ1; cat#801203; Biolegend), Tyrosine hydroxylase (cat#2792; Cell Signaling), B220-APC (RA3-6B2; cat#17-0452-82; eBioscience), anti-rabbit IgG-APC (Jackson; cat# 111-136-144), anti-rabbit IgG-AlexaFluor 488 (cat#A11008; Life Technologies).
Camell C.D., Sander J., Spadaro O., Lee A., Nguyen K.Y., Wing A., Goldberg E.L., Youm Y.H., Brown C.W., Elsworth J., Rodeheffer M.S., Schultze J.L, & Dixit V.D. (2017). Inflammasome-driven catecholamine catabolism in macrophages blunts lipolysis in the aged. Nature, 550(7674), 119-123.
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2

Comprehensive Immunophenotyping of Adipose Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
For flow cytometry analysis, the following antibodies were used: Fixable Viability Dye Aqua; CD45-BV711 (30F11: cat#563709) (BDBioscience); F4/80-eFlour450; F4/80-PE (BM8; cat#48-4801-82 & 12-4801-82); CD11b-PerCPCy5.5 (M1/70; cat#45-0112-82); CD11c-APC-eFluor780 (N418; cat#47-0114-82); B220-APC (RA3-6B2; cat#17-0452-82); MHCII-Alexa Fluor 700 (M5/114.15.2; cat#56-5321-82) (eBioscience) and CD3-BV605 (17A2; cat#100237); CD206-PECy7 (C068C2; cat#141709) (Biolegend). For western blot analysis, the following antibodies were used: pHSL (Ser660; cat#4126), pHSL (Ser563; cat#4139), HSL (cat#4107), ATGL (cat#2439), Actin (cat#4967), UCP-1 (cat#14670), pNFkB (cat#3033), NFkB (cat#8242), (Cell Signaling), Caspase-1 (Genentech). For whole mount staining: F4/80-PE (BM8; 12-4801-82; eBioscience), TUBB3-AlexaFluor488 (TUJ1; cat#801203; Biolegend), Tyrosine hydroxylase (cat#2792; Cell Signaling), B220-APC (RA3-6B2; cat#17-0452-82; eBioscience), anti-rabbit IgG-APC (Jackson; cat# 111-136-144), anti-rabbit IgG-AlexaFluor 488 (cat#A11008; Life Technologies).
Camell C.D., Sander J., Spadaro O., Lee A., Nguyen K.Y., Wing A., Goldberg E.L., Youm Y.H., Brown C.W., Elsworth J., Rodeheffer M.S., Schultze J.L, & Dixit V.D. (2017). Inflammasome-driven catecholamine catabolism in macrophages blunts lipolysis in the aged. Nature, 550(7674), 119-123.
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