Spectra manager suite software

To identify the type of material, infrared spectra were acquired using an ATR (attenuated total reflectance)-FTIR spectrometer (FT-IR 4700, Jasco, Japan) equipped with a diamond optical crystal and controlled by Spectra Manager^TM Suite software (Jasco, Japan). FTIR spectra were acquired in the range 4000–650 cm⁻¹, performing a total of 25 scans with a resolution of 4.0 cm⁻¹.

The secondary structure analysis of the AMS8 lipase was measured using JASCO 815 spectropolarimeter (JASCO, Tokyo, Japan). The secondary structure contents of AMS8 lipase with CaCl₂ were valued based on the far-UV (190–260 nm) using a 1 mm path cell length at 20 °C. Refolded AMS8 lipase protein (1 mg/mL) with varying CaCl₂ concentrations (0 (control), 20, 40, 60, 80 and 100 mM) was prepared in Tris–HCl (1mM, pH 8.0) buffer. The spectral analysis of AMS8 lipase was subtracted with the subsequent blank containing Tris–HCl (1 mM, pH 8.0). All measurements were repeated three times. The secondary structure contents (beta, helix, turn and random) of AMS8 lipase were analyzed using the method by Yang et al. [38 (link)], using Spectra Manager^TM Suite Software (JASCO, Tokyo, Japan).