Nelfinavir mesylate (NFR) powder was extracted and purified from 250 mg tablets (Agouron pharmaceuticals; San Diego, CA). Curcumin (CUR) was obtained from Acros Organics (Fair Lawn, NJ). Docetaxel (DTX) and Thapsigargin (Tg) were obtained from Sigma (St. Louis, MO) and the AKT-inhibitor (AKTi-IV; Catalog no. 124005) was obtained from Calbiochem (Billerica, MA). For in vitro studies, all drugs were dissolved in dimethyl sulfoxide (DMSO). Primary antibodies against total-AKT and phospho-AKT, total eIF2α and phospho-eIF2α, and against human BiP/Grp78, PARP and CHOP, were all purchased from Cell Signaling Technology (Danvers, MA). Antibodies against human TRIB3 and ATF4 were from Santa Cruz Biotechnology (Santa Cruz, CA), antibody against human β-Actin was from Fisher Scientific (Waltham, MA) and against Ki-67 was from Spring Biosciences (Pleasanton, CA). All secondary antibodies, such as goat anti-mouse, goat anti-rabbit and bovine anti-goat, were all purchased from Santa-Cruz Biotechnology.
Curcumin cur
Manufactured by Thermo Fisher Scientific
Sourced in United States, Singapore
Curcumin (Cur) is a natural compound derived from the turmeric plant. It is a yellow-colored polyphenol with known antioxidant and anti-inflammatory properties. Curcumin is commonly used in laboratory research as a reference standard and in the development of analytical methods.
Automatically generated - may contain errors
Lab products found in correlation
Lysotracker green, by Thermo Fisher Scientific (2 mentions)
Of siccat1 or cyanine 3 cy3 labeled siccat1, by RiboBio (2 mentions)
Antibodies of western bolt, by Cell Signaling Technology (2 mentions)
Fetal bovine serum (fbs), by Wisent (2 mentions)
Rnase free deionized water, by Tiangen Biotech (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Docetaxel dtx, by Merck Group (1 mentions)
Phospho eif2α, by Cell Signaling Technology (1 mentions)
Thapsigargin tg, by Merck Group (1 mentions)
Goat anti mouse, by Santa Cruz Biotechnology (1 mentions)
Eif2α, by Cell Signaling Technology (1 mentions)
Bovine anti goat, by Santa Cruz Biotechnology (1 mentions)
Goat anti rabbit, by Santa Cruz Biotechnology (1 mentions)
Sodium alginate, by Loba Chemie (1 mentions)
Milli q purification system, by Merck Group (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
0.8 μm polycarbonate membrane, by Merck Group (1 mentions)
1 2 distearoyl sn glycero 3 phosphoethanolamine n succinyl polyethylene glycol 2000 dspe peg cooh, by Avanti Polar Lipids (1 mentions)
1 2 dipalmitoyl sn glycero 3 phosphocholine dppc, by Avanti Polar Lipids (1 mentions)
8 protocols using curcumin cur
1
Nelfinavir Mesylate Purification Protocol
2
Curcumin-Loaded Alginate-Whey Protein Hydrogels
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Sodium alginate (91%) was obtained from Loba Chemie (Mumbai, India). The whey protein isolate (WPI) used herein was of 90% purity. Curcumin (CUR, 98%) was obtained from Acros Organics (Mumbai, India). 2,2-diphenyl-2-picrylhydrazyl (DPPH, 95%) was purchased from Thermo Fisher Scientific (Geel, Belgium). Ethanol (98.0%), glycerol (GLY, 99.5%), and citric acid (CA, 99%) were of analytical grade and obtained from commercial sources.
3
Curcumin-loaded PEI-based Nanoparticles
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Branched polyethyleneimine (PEI, 25 kDa) was obtained from Sigma-Aldrich (MO, USA). d , l -lactide (DLLA) was obtained from Alfa Aesar (MA, USA). The anhydrous dimethyl sulfoxide (DMSO) was purchased from J&K Scientific Ltd. 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)2000] (DSPE-mPEG) was obtained from A.V.T. (Shanghai) Pharmaceutical Co., Ltd. Curcumin (Cur) was purchased from Alfa Aesar (MA, USA). The 0.8 μm polycarbonate membrane was purchased from Millipore Co. (MA, USA). The mimics of siCCAT1 or cyanine-3 (Cy3) labeled siCCAT1 were obtained from RiboBio Co. (Guangzhou, China). Antibodies of western bolt were purchased from Cell Signaling Technology, Inc and Abcam plc. RNase-free deionized water was purchased from TIANGEN Biotech Co. Ltd (Beijing). LysoTracker Green was obtained from Molecular Probes Inc. (Eugene, OR). The cell counting kit-8 (CCK-8) was obtained from Dojindo Molecular Technologies, Inc., (Japan). Fetal bovine serum (FBS) was obtained from Wisent Inc. 0.25% trypsin–EDTA, RPMI 1640 medium and penicillin/streptomycin were obtained from Thermo Fisher Scientific (MA, USA). The water used was of ultrapure grade and was supplied by a Milli-Q purification system of Millipore Co. (MA, USA).
4
Curcumin-loaded PLGA-PEG Nanoparticles
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Poly(D, L-lactide-co-glycolide) acid-terminated (PLGA, lactide:glycolide 50:50, Mw 38,000–54,000), was purchased from Sigma-Aldrich (St. Louis, MO, USA). Curcumin (Cur) was bought from AlfaAesar (Haverhill, MA, USA). 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[succinyl(polyethylene glycol)-2000] (DSPE-PEG-COOH) and 1,2-Dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) were purchased from Avanti Polar Lipid (Alabaster, AL, USA). All reagents and solvents were used without further purification.
5
Curcumin Protein Interaction Assay
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Curcumin (CUR) (≥95 wt.% curcuminoid) was purchased from Alfa Aesar (Singapore, Singapore). Bovine serum albumin (BSA) with a molecular weight (MW) of 66.5 kDa, potassium hydroxide (KOH), glacial acetic acid (AA), phosphate-buffered saline (PBS, pH 6.8), sodium dodecyl sulfate (SDS), ethanol, acetonitrile, trifluoroacetic acid (TFA), and sodium chloride (NaCl) were purchased from Sigma Aldrich (Singapore, Singapore). Sucrose (≥99% wt.%) was purchased from Tokyo Chemical Industry (Tokyo, Japan). In addition, 30% Acrylamide/Bis-acrylamide (29:1 w/w), tris, sodium dodecyl sulfate, ammonium persulfate, N,N,N′,N′-Tetramethyl ethylenediamine (TEMED), ß-mercaptoethanol, Coomassie Brilliant Blue G-250, and an SDS-PAGE sample loading buffer were purchased from Bio-Rad (Singapore, Singapore). A PageRuler™ prestained protein ladder (15 to 250 kDa) was purchased from Thermo Scientific (Singapore, Singapore).
6
Curcumin-Loaded DSPE-mPEG Nanoparticles
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Branched polyethyleneimine (PEI, 25 kDa) was obtained from Sigma-Aldrich (MO, USA). D, L-Lactide (DLLA) was obtained from Alfa Aesar (MA, USA). The anhydrous dimethyl sulfoxide (DMSO) was purchased from J&K Scienti c Ltd. 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)2000] (DSPE-mPEG) was obtained from A.V.T.(Shanghai) Pharmaceutical Co., Ltd. Curcumin (Cur) was purchased from Alfa Aesar (MA, USA). The 0.8 µm polycarbonate membrane was purchased from Millipore Co. (MA, USA). The mimics of siCCAT1 or cyanine-3 (Cy3) labeled siCCAT1 were obtained from RiboBio Co. (Guangzhou, China). Antibodies of western bolt were purchased from Cell Signaling Technology, Inc and Abcam plc. RNase-free deionized water was purchased from TIANGEN Biotech Co. Ltd (Beijing). LysoTracker Green was obtained from Molecular Probes Inc. (Eugene, OR). The cell counting kit-8 (CCK-8) was obtained from Dojindo Molecular Technologies, Inc., (Japan). Fetal bovine serum (FBS)was obtained from Wisent Inc. 0.25% trypsin-EDTA, RPMI 1640 medium and penicillin/streptomycin were obtained from Thermo Fisher Scienti c (MA, USA).
The water used was of ultrapure grade and was supplied by a Milli-Q puri cation system of Millipore Co. (MA, USA).
The water used was of ultrapure grade and was supplied by a Milli-Q puri cation system of Millipore Co. (MA, USA).
7
Curcumin-loaded PLA/HPbCD Nanoparticles
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Polylactic acid (PLA) (Natureworks, product code 6252D) and hydroxypropyl-beta-cyclodextrin (HPbCD) (Wacker Chemie AG, Germany) was donated to our research group for laboratory studies. Curcumin (CUR, !95%, Alfa Aesar), zinc acetate dehydrate (Sigma Aldrich), fluorescein isothiocyanate (FITC, Sigma Aldrich), potassium phosphate monobasic (Sigma Aldrich), sodium phosphate dibasic heptahydrate (Sigma Aldrich), sodium chloride (Sigma Aldrich), methanol (extra pure, Sigma Aldrich), chloroform (CHCl 3 , extra pure, Sigma Aldrich), deuterated dimethylsulfoxide (DMSO-d6, deuteration degree min 99.8% for NMR spectroscopy, Merck), hydrochloric acid (HCl, 36.5-38%, Sigma-Riedel), 2,2diphenyl-1-picrylhydrazyl (DPPH, Sigma Aldrich) were purchased and used as-received without any further purification. Distilleddeionized water was supplied from Millipore milli-Q ultrapure water system.
8
Curcumin-Chitosan Nanoparticle Cytotoxicity
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Curcumin (CUR) (95% curcuminoid) was purchased from Alfa Aesar (Singapore). Chitosan (CHI) (50-190 kDa, 75-85% deacetylation), hypromellose (hydroxypropylmethylcellulose, HPMC) (26 kDa), potassium and sodium hydroxides (KOH, NaOH), potassium dihydrogen phosphate (KH2PO4), sodium chloride (NaCl), hydrogen chloride (HCl), glacial acetic acid (AA), and ethanol were purchased from Sigma-Aldrich (Singapore). The materials and methods for the cytotoxicity test were provided in the Supplementary Materials.
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