The rapeseed protein-derived peptides Thr-Phe (TF), Leu-Tyr (LY) and Arg-Ala-Leu-Pro (RALP) were synthesized (>95% purity) by GenWay Biotech (GenWay Biotech Inc. San Diego, CA). Human recombinant renin protein (10006217; >99% purity) and renin inhibitor screening assay kit (10006270) were purchased from Cayman Chemicals (Ann Arbor, MI). Rabbit lung ACE (A6778, 98% purity) and N-[3-(2-Furyl) acryloyl]-L-phenylalanyl-glycyl-glycine (FAPGG) were purchased from Sigma-Aldrich (St. Louis, MO). Other analytical grade reagents were obtained from Fisher Scientific (Oakville, ON, Canada).
Rabbit lung ace
Manufactured by Merck Group
Sourced in United States
Rabbit lung ACE is a laboratory equipment product used in research applications. It functions as a tool to measure the activity of angiotensin-converting enzyme (ACE) in rabbit lung tissue samples.
Automatically generated - may contain errors
Lab products found in correlation
Quercetin, by Merck Group (3 mentions)
Trolox, by Merck Group (3 mentions)
Hippuryl histidyl leucine, by Merck Group (3 mentions)
Gallic acid, by Merck Group (3 mentions)
N 3 2 furyl acryloyl l phenylalanyl glycyl glycine fapgg, by Merck Group (2 mentions)
Acarbose, by Merck Group (2 mentions)
Orlistat, by Merck Group (2 mentions)
Porcine pl, by Merck Group (2 mentions)
Pepsin, by Merck Group (2 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (2 mentions)
Porcine pancreatic lipase, by Merck Group (2 mentions)
Catechin, by Merck Group (2 mentions)
Caffeic acid, by Merck Group (2 mentions)
Chlorogenic acid, by Merck Group (2 mentions)
Ellagic acid, by Merck Group (2 mentions)
Methanol, by Merck Group (2 mentions)
Rutin, by Merck Group (2 mentions)
Apigenin, by Merck Group (2 mentions)
Luteolin, by Merck Group (2 mentions)
Epicatechin, by Merck Group (2 mentions)
7 protocols using rabbit lung ace
1
Rapeseed Peptides' Renin and ACE Inhibition
2
Evaluation of Anti-Diabetic Potential of Bangladeshi Vegetables
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Fresh vegetables, namely ash gourd (BINA CHALKUMRA 1), bitter gourd (BARI KOROLA 1), brinjal (BARI BEGUN 8), Indian spinach (BARI PUISHAK 1), kangkong (BARI GIMAKOLMI 1), okra (BARI DHEROSH 2), ridge gourd (BARI JHINGA 1), snake gourd (BARI CHICHINGA 1), and stem amaranth (BARI DATA 1), were collected from the Bangladesh Agricultural Research Institute (BARI), Gazipur, Bangladesh. The vegetables were dried in the oven at 40 °C, ground into powders, and then stored at −20 °C. Porcine pancreatic α-amylase, porcine PL, rat intestinal acetone powder, acarbose, orlistat, 4-nitrophenyl α-D-glucopyranoside (PNP), N-(3-[2-furyl]acryloyl)-phenylalanyl glycyl glycine (FAPGG), and rabbit lung ACE were purchased from Sigma-Aldrich (St. Louis, MO, USA). A human recombinant renin inhibitor screening assay kit was purchased from Cayman Chemicals (Ann Arbor, MI, USA). Other analytical grade chemical reagents were obtained from Fisher Scientific Company (Oakville, ON, Canada).
3
Enzymatic Hydrolysis of Caribbean Seal
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R. canadum was purchased from Tan Hou Ocean Development Co. (Penghu, Taiwan), Taipei. Protamex (a mixture of endo and exopeptidase from Bacillus sp., activity labelled 1.5 AU/g) and Protease N (a mixture of endo and exopeptidase from Bacillus subtilis, activity labelled 150,000 U/g) were respectively obtained from Novo Nordisk A/S Co. (Bagsværd, Denmark) and Amano Pharmaceutical Co. (Yokohama, Kanagawa, Japan). Enzymes including pepsin, pancreatin and rabbit lung ACE were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Substrate (hippuryl-L-histidyl-L-leucine, HHL) and all chemicals used were of analytical grade and also purchased from Sigma Chemical Co. (St. Louis, MO, USA).
4
Phytochemical Evaluation and Bioactivity
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The chemicals used in this study, including Folin–Ciocalteu reagent, gallic acid, Folin-Denis reagent, tannic acid, aluminum chloride, quercetin, ABTS [2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)], K2S2O8 (potassium persulfate), phosphate buffer, potassium ferricyanide, trichloroacetic acid, ferric chloride, DPPH* (2,2-diphenyl-1-picrylhydrazyl), rabbit lung ACE, porcine pancreatic lipase, α-amylase, Bacillus stearother-mophillus α-glucosidase, hippuryl-histidyl-leucine, orlistat, acarbose, captopril, and Trolox, were products of Sigma (St. Louis, USA). These and other chemicals and reagents used for the various analyses in this study were of analytical purity.
5
Renin Inhibitor Screening Assay
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The Renin Inhibitor Screening Assay Kit was purchased from Cayman Chemicals (Ann Arbor, MI, USA). Enzeco Trypsin-Chymotrypsin® 1:1 was obtained from Enzyme Development Corporation, New York, NY, USA. Pepsin, rabbit lung ACE, and N-[3-(2-furyl) acryloyl]-l-phenylalanyl-glycyl-glycine (FAPGG) were purchased from Sigma Chemicals (St. Louis, MO, USA). Methanol (HPLC grade), trifluoroacetic acid (TFA), 0.45- and 0.2-micron syringe filters, and all other analytical grade chemical reagents were obtained from Fisher Scientific (Oakville, ON, Canada).
6
Bioactive Compound Characterization
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Methanol, formic acid, gallic acid, chlorogenic acid, caffeic acid, and ellagic acid purchased from Merck (Darmstadt, Germany). Catechin, epiCatechin, quercetin, rutin, apigenin, and luteolin; porcine pancreatic lipase, Hippuryl-histidyl- leucine (Bz-Gly-His-Leu), Rabbit lung ACE, Xanthine, Allopurinol, 2,2-diphenylpicrylhydrazyl (DPPH), 2,2´-azino-bis-3-ethylbenzthiazoline-6-sulphoni (ABTS), and Trolox, L-ascorbic acid (Vitamin C) were acquired from Sigma Chemical Co. (St. Louis, MO, USA). All other chemicals used for analysis were of analytical grade.
7
Quantification of Bioactive Compounds
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Methanol, formic acid, gallic acid, chlorogenic acid, caffeic acid, and ellagic acid were purchased from Merck (Darmstadt, Germany). Catechin, epiCatechin, quercetin, rutin, apigenin, and luteolin; porcine PL, Hippuryl-histidyl-leucine, Rabbit lung ACE, DPPH, ABTS, and Trolox were acquired from Sigma Chemical Co. (St. Louis, MO, USA). All other chemicals used for the analysis were of analytical grade.
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