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2 protocols using bupropion

1

Cell Culture and Viability Assay Protocol

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Vs, Hs and Vn were purchased from Sigma Aldrich, USA. Dulbecco’s Modified Eagle Medium (DMEM)/F-12 and Opti-MEM were obtained from Gibco, USA. Fetal bovine serum was from Gibco, South America. Penicillin - Streptomycin were purchased from Biowhittaker, USA. Non-essential amino acids were from Cosmo Bio Co, LTD, Japan. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and dexamethasone were from Dojindo, Japan. Bupropion was from Wako, Japan. ATP bioluminescence kit was from TOYO Ink, Japan. ISOGEN kit was purchased from Nippon Gene, Japan. RIPA lysis buffer was from (Santa Cruz Biotechnology, USA). 2-D Quant was purchased from GE Healthcare Life Sciences, USA. Calcium Kit II-Fluo 4 was from Dojindo, Japan.
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2

Chemicals and Reagents for Neuroscience Research

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Ethylenediamine-N,N,N',N'-tetraacetic acid (EDTA) was obtained from Dojindo (Kumamoto, Japan). Fluoxetine, imipramine, venlafaxine, bupropion, and other chemicals were obtained from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan).
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