Recombinant human cx3cl1

Recombinant human CX3CL1 was obtained from Peprotech. Recombinant human IL-6 protein was obtained from R&D systems. Acriflavine (HIF-1 inhibitor), KN-93 (CaMKII inhibitor), GSK690693 (pan-Akt inhibitor), BAY 11-7082 (NF-κB signaling inhibitor), U73122 hydrate (pan-PLC inhibitor), Stattic (STAT3 inhibitor), LY-294002 hydrochloride (PI3K inhibitor), Sodium oxamate (LDHA inhibitor), pertussis toxin (PTx,Gα_i/0 inhibitor), H-89 (PKA/ROCK inhibitor), Y-27632 (ROCK inhibitor) and CoCl₂ were all obtained from Sigma-Aldrich. Rapamycin (mTORC1 inhibitor) was purchased from Cayman Chemical. 17-AAG (HSP90 inhibitor) and P6 (pan-JAK inhibitor), Bisindolylmaleimide I (PKC inhibitor) were purchased from Calbiochem. The Gα_q-inhibitor UBO-QIC was purchased from the Institute of Pharmaceutical Biology, University of Bonn. Gallein (βγ-subunit inhibitor) was obtained from Tocris.

Recombinant human CX3CL1 (cat#300-31) was from Peprotech; IL-4 (cat#12340045) was from Immunotools; LPS (cat#L4391) was from Sigma-Aldrich; anti-Arg1 antibody was from Santa Cruz (cat#sc-271430 RRID:AB_10648473); anti-Actin antibody (cat#A2066) was from Sigma-Aldrich. Secondary antibodies were from DAKO; Microbeads CD11b+ were from Miltenyi Biotec. All cell culture media, fetal bovine serum (FBS), goat serum, penicillin G, streptomycin, glutamine and Hoechst (cat#33342, RRID:AB_10626776) were from Invitrogen; poly-L-lysine (cat#P2636) and papain were from Sigma-Aldrich. Griess reagent kit for Nitrite determination was from Molecular Probe (cat#G-7921), Lactate Assay Kit (cat#MAK064) and Arginase Activity Assay Kit (cat#MAK112) were from Sigma-Aldrich; Seahorse Cell Mito Stress Test Kit for Seahorse XFe Analyzer Respiratory Assay reagents were from Agilent (cat#103015-100).