All chemicals were purchased without further purification. Dequalinium chloride, oligomycin, adenosine 5′-triphosphate (ATP) disodium salt hydrate, cytidine 5′-triphosphate disodium salt (CTP), guanosine 5′-triphosphate sodium salt hydrate (GTP), uridine 5′-triphosphate tris salt (UTP), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT), sodium dodecyl sulfate (SDS), deoxycholic acid, phenylmethylsulfonyl fluoride (PMSF), and ethidium bromide (EB) were purchased from Sigma-Aldrich. PMSF Protease Inhibitor, MitoTracker Green, methanol, dimethyl sulfoxide (ACS certified) and JC-1 dye were purchased from Fisher Scientific. Phosphate-buffered saline (PBS) was purchased from Corning and Opti-MEM was purchased from Gibco. Dulbecco's modification of Eagle's medium (DMEM) was prepared by Dr Sandra McMasters of the University of Illinois School of Chemical Sciences Cell Media Facility. All experiments were performed at room temperature unless mentioned otherwise.
Cytidine 5 triphosphate disodium salt
Manufactured by Merck Group
Sourced in United States
Cytidine 5'-triphosphate disodium salt is a chemical compound used as a laboratory reagent. It serves as a precursor in the synthesis of nucleic acids and as a substrate for various enzymatic reactions.
Automatically generated - may contain errors
Lab products found in correlation
Guanosine 5 triphosphate sodium salt hydrate, by Merck Group (2 mentions)
Adenosine 5 triphosphate, by Merck Group (2 mentions)
Deoxycholic acid, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions)
Oligomycin, by Merck Group (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
Ethidium bromide, by Merck Group (1 mentions)
Jc 1 dye, by Thermo Fisher Scientific (1 mentions)
Dequalinium chloride, by Merck Group (1 mentions)
Pmsf protease inhibitor, by Thermo Fisher Scientific (1 mentions)
Mitotracker green, by Thermo Fisher Scientific (1 mentions)
Opti mem, by Thermo Fisher Scientific (1 mentions)
Sodium dodecyl sulfate (sds), by Merck Group (1 mentions)
Phenylmethylsulfonyl fluoride, by Merck Group (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Corning (1 mentions)
Adenosine 5 triphosphate disodium salt hydrate, by Merck Group (1 mentions)
2 deoxyglucose 2 dg, by Merck Group (1 mentions)
Malachite green, by Merck Group (1 mentions)
Ammonium molybdate, by Merck Group (1 mentions)
4 protocols using cytidine 5 triphosphate disodium salt
1
Mitochondrial Dysfunction Assays
2
ATP-Binding DNA Aptamer Beacon Probe
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The novel DNA aptamer beacon probe, designed for recognition of ATP, consisted of a 37-mer oligonucleotide strand and a fluorophore-quencher pair (FAM-Dabcyl) attached to the ends of the strand with the following sequence: 5′-FAM-ATTCTCTCACCTGGGGGAGTATTGCGGAGGAAGGTAT-Dabcyl-3′. It was synthesized by the FUTUREsynthesis Sp. z o.o. Poznan, Poland. The purity of this oligonucleotide was tested with HPLC. Adenosine 5′-triphosphate disodium salt hydrate (ATP), guanosine 5′-triphosphate sodium salt hydrate (GTP), cytidine 5′-triphosphate disodium salt (CTP), uridine 5′-triphosphate trisodium salt dihydrate (UTP), trizma hydrochloride (Tris-HCl), magnesium chloride (MgCl2), sodium chloride (NaCl), and 2-deoxyglucose (2-DG) were obtained from Sigma-Aldrich Chemical Company (St. Louis, MO, USA). All chemicals were of analytical grade purity. Aqueous solutions were prepared with freshly deionized water with 18.2 MΩ cm resistivity (Millipore, Poland). All concentrations of added reagents cited in this paper are final concentrations obtained after mixing, unless otherwise noted.
3
Quantification of Nucleotide Levels
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All reagents were of analytical grade. Bovine serum albumin (BSA), malachite green, adenosine 5′ -triphosphate (ATP), adenosine 5′ -diphosphate (ADP), cytidine 5′-triphosphate disodium salt (CTP), adenosine 5′ -monophosphate (AMP), uridine 5’-triphosphate (UTP), uridine 5’-diphosphate (UDP), guanosine-5’-triphosphate (GTP), phosphate-buffered saline (DPBS), 4-(2-hydroxyethyl)-1-piperazineetahnesulfonic acid (HEPES), ammonium molybdate, Triton X-100, phenylmethylsulphonyl fluoride (PMSF), pyruvate kinase, phosphoenol-pyruvate (PEP), luciferase, coenzyme A and P1,P5-Di (adenosine-5´) pentaphosphate pentasodium salt (Ap5A) were purchased from Sigma-Aldrich (St Louis, MO, USA). D-luciferin was purchased from Molecular Probes Inc. (Eugene, OR, USA).
4
ATP-Binding DNA Aptamer Synthesis
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Fluorescein (FAM)-labeled DNA aptamer for the recognition of ATP with the following sequence 5′-TCTCTCACCTGGGGGAGTATTGCGGAGGAAGGT-FAM-3′ has been synthesized by the Laboratory of DNA Sequencing and Oligonucleotides Synthesis, Institute of Biochemistry and Biophysics of the Polish Academy of Sciences (IBB PAS, Warsaw, Poland). The purity of this oligonucleotide was tested with HPLC. Oligomycin A, adenosine 5′-triphosphate disodium salt hydrate grade I (ATP), guanosine triphosphate (GTP), cytidine 5′-triphosphate disodium salt (CTP), uridine 5′-triphosphate trisodium salt dehydrate (UTP), trizma hydrochloride (Tris-HCl), hydrogen peroxide solution (H2O2), magnesium chloride (MgCl2), carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP) and sodium chloride (NaCl), 0.25% Trypsin-EDTA solution were obtained from Sigma-Aldrich Chemical Company (St. Louis, MO, USA).
Fetal bovine serum (FBS) was obtained from Gibco (Rockville, MD, USA). Penicillin/streptomycin and Dulbecco’s modified Eagle’s Medium (DMEM) were obtained from the cell culture company PAA (Immuniq, Warsaw, Poland). All chemicals were of analytical grade purity. Aqueous solutions were prepared with freshly deionized water with 18.2 MΩ cm resistivity (Millipore, Poland). All concentrations of added reagents cited in this paper are final concentrations obtained after mixing, unless otherwise noted.
Fetal bovine serum (FBS) was obtained from Gibco (Rockville, MD, USA). Penicillin/streptomycin and Dulbecco’s modified Eagle’s Medium (DMEM) were obtained from the cell culture company PAA (Immuniq, Warsaw, Poland). All chemicals were of analytical grade purity. Aqueous solutions were prepared with freshly deionized water with 18.2 MΩ cm resistivity (Millipore, Poland). All concentrations of added reagents cited in this paper are final concentrations obtained after mixing, unless otherwise noted.
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