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Inverted infinity and phase contrast microscope

Manufactured by Thermo Fisher Scientific
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The Inverted Infinity and Phase Contrast Microscope is a lab equipment designed for accurate and detailed microscopic observation. It features an inverted optical design and phase contrast capabilities to enhance contrast and visibility of transparent specimens. The core function of this microscope is to provide clear and detailed images for various scientific and research applications.

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Lab products found in correlation

Facsaria 2 system, by BD (2 mentions) Hsc003 methylcellulose medium, by R&D Systems (2 mentions) Methocult m3434, by STEMCELL (2 mentions) Sc 26196, by Merck Group (2 mentions) Methl pyruvate, by Merck Group (2 mentions) Facsaria 2, by BD (1 mentions) Rhscf, by Gemini Bio (1 mentions) Rhtpo, by Gemini Bio (1 mentions) Myelocult h5100 medium, by STEMCELL (1 mentions) Eltrombopag, by Novartis (1 mentions)

Spelling variants of this product

Inverted microscope (22 citations) Phase Contrast Microscope (5 citations) Inverted Infinity (3 citations)

6 protocols using inverted infinity and phase contrast microscope

1

Assessing Stem Cell Differentiation Potential

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To assess differentiation potential of phenotypic pre-malignant stem
cells (preMDS/AML-SC) and malignant stem cells (MDS/AML-SCs), cells were
FACS-sorted from additional patients with the same strategy (Supplementary Fig. 1a), and plated
in HSC003 methylcellulose medium according to the manufacturer’s
recommendation (R&D Systems, Minneapolis, MN). Colonies of different
hematopoietic lineages were scored two weeks after plating using an Inverted
Infinity and Phase Contrast Microscope (Fisher Scientific, Hampton, NH). In
addition, to examine the expression of lineage makers, methylcellulose medium
was dissolved in PBS to dissociate the colonies into single cell suspension.
Cells were stained with antibodies against CD14, CD15 and CD235a on ice for 30
minutes, then analyzed on a BD FACSAria II system.
Chen J., Kao Y.R., Sun D., Todorova T.I., Reynolds D., Narayanagari S.R., Montagna C., Will B., Verma A, & Steidl U. (2018). Myelodysplastic Syndrome Progression to Acute Myeloid Leukemia at the Stem Cell Level. Nature medicine, 25(1), 103-110.
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2

Biodistribution of ZnS:Ag,Co@ZnS Nanoparticles

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On day 3 and day 28 after i.v. administration of ZnS:Ag,Co@ZnS nanoparticles, injected mice were euthanized and their major organs (brain, heart, lung, liver, spleen, and kidney) were harvested and fixed in 4% paraformaldehyde. After 48 h of tissue fixation, these organs were embedded in paraffin and sectioned to 10-µm slices. Later, the slices were stained with hematoxylin and eosin (H&E), followed by imaging under an inverted infinity and phase-contrast microscope (Fisher Scientific).
Wu X., Zhu X., Chong P., Liu J., Andre L.N., Ong K.S., Brinson K J.r., Mahdi A.I., Li J., Fenno L.E., Wang H, & Hong G. (2019). Sono-optogenetics facilitated by a circulation-delivered rechargeable light source for minimally invasive optogenetics. Proceedings of the National Academy of Sciences of the United States of America, 116(52), 26332-26342.
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3

Assessing Stem Cell Differentiation Potential

Check if the same lab product or an alternative is used in the 5 most similar protocols
To assess differentiation potential of phenotypic pre-malignant stem
cells (preMDS/AML-SC) and malignant stem cells (MDS/AML-SCs), cells were
FACS-sorted from additional patients with the same strategy (Supplementary Fig. 1a), and plated
in HSC003 methylcellulose medium according to the manufacturer’s
recommendation (R&D Systems, Minneapolis, MN). Colonies of different
hematopoietic lineages were scored two weeks after plating using an Inverted
Infinity and Phase Contrast Microscope (Fisher Scientific, Hampton, NH). In
addition, to examine the expression of lineage makers, methylcellulose medium
was dissolved in PBS to dissociate the colonies into single cell suspension.
Cells were stained with antibodies against CD14, CD15 and CD235a on ice for 30
minutes, then analyzed on a BD FACSAria II system.
Chen J., Kao Y.R., Sun D., Todorova T.I., Reynolds D., Narayanagari S.R., Montagna C., Will B., Verma A, & Steidl U. (2018). Myelodysplastic Syndrome Progression to Acute Myeloid Leukemia at the Stem Cell Level. Nature medicine, 25(1), 103-110.
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4

Single Human HSC Maintenance and Expansion

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Single human HSCs (Lin−CD34+CD38−CD49f+) were sorted with FACS Aria II (Becton Dickinson) and directly deposited onto 60-well Terasaki plate (Thermo Fisher Scientific). Sorted cells were cultured in Myelocult H5100 medium (STEMCELL Technologies) supplemented with 100 ng/ml rhSCF (Gemini Bio-Products) and 10−6 M hydrocortisone (Acros Organics) supporting stem cell maintenance, along with treatment with vehicle control (sterile H2O), eltrombopag (3 or 10 μg/ml, Novartis), or rhTPO (100 ng/ml, Gemini Bio-Products). Single cell deposition in each well was verified 4 hours after seeding, and cells/well were counted again at 24, 48, 72, 96, and 120 hours after treatment using an Inverted Infinity and Phase Contrast Microscope (Fisher Scientific).
Kao Y.R., Chen J., Narayanagari S.R., Todorova T.I., Aivalioti M.M., Ferreira M., Ramos-Marques P., Pallaud C., Mantzaris I., Shastri A., Bussel J.B., Verma A., Steidl U, & Will B. (2018). Thrombopoietin receptor-independent stimulation of hematopoietic stem cells by eltrombopag. Science translational medicine, 10(458), eaas9563.
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5

Hematopoietic Stem Cell Clonogenic Assay

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Sorted mouse HSC (180 cells Lin−/cKit+/Sca1+/CD48−/CD150+) or CD34-enriched human HSPC (5,000 cells) were plated into methylcellulose media (STEMCELL Technologies, MethoCult M 3434 for mouse or MethoCult H4434 for human) according to the manufacturer’s protocols. Colonies were scored after 10 to 12 days for mouse and 14 to 16 days for human using an Inverted Infinity and Phase Contrast Microscope (Fisher Scientific). For serial re-plating assays, methylcellulose medium from primary platings were dissolved in PBS to dissociate the colonies into a single-cell suspension, washed three times and 20,000 cells (mouse) or 40,000 cells (human) were re-plated in 1ml of MethoCult M3434 or MethoCult H4434 medium for mouse and human cells, respectively. Where indicated, media was supplemented with: 100 nM FADS2 inhibitor SC-26196 (Sigma, PZ0176), 100 μM γ-linolenic fatty acid (GLA) (Sigma, L2378), 100 μM NAC (Sigma, A7250) or 5 mM Methl-pyruvate (Sigma, 371173).
Dong S., Wang Q., Kao Y., Diaz A., Tasset I., Kaushik S., Thiruthuvanathan V., Zintiridou A., Nieves E., Dzieciatkowska M., Reisz J., Gavathiotis E., D’Alessandro A., Will B, & Cuervo A. (2021). Chaperone-mediated autophagy sustains hematopoietic stem cell function. Nature, 591(7848), 117-123.
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6

Hematopoietic Stem Cell Clonogenic Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sorted mouse HSC (180 cells Lin−/cKit+/Sca1+/CD48−/CD150+) or CD34-enriched human HSPC (5,000 cells) were plated into methylcellulose media (STEMCELL Technologies, MethoCult M 3434 for mouse or MethoCult H4434 for human) according to the manufacturer’s protocols. Colonies were scored after 10 to 12 days for mouse and 14 to 16 days for human using an Inverted Infinity and Phase Contrast Microscope (Fisher Scientific). For serial re-plating assays, methylcellulose medium from primary platings were dissolved in PBS to dissociate the colonies into a single-cell suspension, washed three times and 20,000 cells (mouse) or 40,000 cells (human) were re-plated in 1ml of MethoCult M3434 or MethoCult H4434 medium for mouse and human cells, respectively. Where indicated, media was supplemented with: 100 nM FADS2 inhibitor SC-26196 (Sigma, PZ0176), 100 μM γ-linolenic fatty acid (GLA) (Sigma, L2378), 100 μM NAC (Sigma, A7250) or 5 mM Methl-pyruvate (Sigma, 371173).
Dong S., Wang Q., Kao Y., Diaz A., Tasset I., Kaushik S., Thiruthuvanathan V., Zintiridou A., Nieves E., Dzieciatkowska M., Reisz J., Gavathiotis E., D’Alessandro A., Will B, & Cuervo A. (2021). Chaperone-mediated autophagy sustains hematopoietic stem cell function. Nature, 591(7848), 117-123.
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