C ebpδ

Manufactured by Abcam

Sourced in United Kingdom

C/EBPδ is a transcription factor that regulates gene expression involved in cell differentiation, inflammation, and metabolism. It is a member of the CCAAT/enhancer-binding protein (C/EBP) family.

Automatically generated - may contain errors

Lab products found in correlation

C ebpβ, by Abcam (2 mentions) Goat serum, by Thermo Fisher Scientific (1 mentions) Anti ly6g clone 1a8, by BD (1 mentions) Normal rabbit igg, by Abcam (1 mentions) Evos fl microscope, by Thermo Fisher Scientific (1 mentions) Foxp3 staining kit, by Thermo Fisher Scientific (1 mentions) Anti cd45 clone 30 f11, by Thermo Fisher Scientific (1 mentions) Rabbit monoclonal igg, by Abcam (1 mentions) Ghost dye, by Thermo Fisher Scientific (1 mentions) Anti ly6c clone hk1, by Thermo Fisher Scientific (1 mentions) Anti cd11b clone m1 70, by BioLegend (1 mentions) Enhanced chemiluminescence, by GE Healthcare (1 mentions) Enzyme linked immunosorbent assay kit, by Thermo Fisher Scientific (1 mentions) Histone h3, by Cell Signaling Technology (1 mentions)

2 protocols using c ebpδ

Multiparameter Analysis of Kidney Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Frozen sections (8 μm) were fixed in 100% methanol and blocked with 5% goat serum (500622, Life Technologies) in Triton X. Primary antibodies were incubated overnight: C/EBPδ (Abcam, 1:200), normal rabbit IgG (Abcam, 1:200), C/EBPβ (Abcam, 1:200) and rabbit monoclonal IgG (Abcam, 1:200). Slides were incubated with goat anti-Rabbit Cy3 antibody (A15020, Thermo Fisher) or DAPI. Slides were visualized on an EVOS FL microscope (Life Technologies).
For flow cytometry, kidneys were harvested following perfusion with PBS. Cells were digested with collagenase IV (1mg/mL) in HBSS. Antibodies: anti-CD45 (clone 30-F11, Thermo Fisher), anti-Ly6G (clone 1A8, BD Biosciences), anti-Ly6C (clone HK1.4, eBioscience), anti-CD11b (clone M1/70, BioLegend), anti-CD133 (clone 13A4, Thermo Fisher), anti-C/EBPδ (Abcam, 1:500), rabbit polyclonal Abs (Abcam, 1:500), and secondary goat anti-Rabbit Alexa Fluor 488 Abs (Thermo Fisher). Dead cells were excluded using Ghost Dye (eBioscience). C/EBPδ intracellular staining was performed with the FOXP3 staining kit (eBioscience). Data acquired with LSR Fortessa and analyzed using FlowJo software (TreeStar).

Bechara R., Amatya N., Bailey R.D., Li Y., Aggor F.E., Li D.D., Jawale C.V., Coleman B.M., Dai N., Gokhale N.S., Taylor T.C., Horner S.M., Poholek A.C., Bansal A., Biswas P.S, & Gaffen S.L. (2021). The m6A reader IMP2 directs autoimmune inflammation through an IL-17- and TNFα-dependent C/EBP transcription factor axis. Science immunology, 6(61), eabd1287.

+ Open protocol

+ Expand

Cytokine and Transcription Factor Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The concentration of IL-6 and TNF-α in culture media was analyzed using enzyme-linked immunosorbent assay kits (eBioscience, San Diego, CA, USA). The expression of transcription factors in the total cell lysate or nuclear lysate was analyzed by western blot using antibodies against C/EBP-β (Abcam, Cambridge, UK), C/EBP-δ (Abcam), ATF3 (Sigma), histone H3 (Cell Signaling, Danvers, MA, USA), and actin (Bethyl Laboratories, Montgomery, TX, USA), with enhanced chemiluminescence (GE Healthcare Life Sciences, Uppsala, Sweden).

Woo M.Y., Yun S.J., Lee M.J., Kim K., Lee E.S, & Park S. (2017). Transcription Factors Regulating Inflammatory Cytokine Production Are Differentially Expressed in Peripheral Blood Mononuclear Cells of Behçet Disease Depending on Disease Activity. Annals of Dermatology, 29(2), 173-179.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!