Cells were cocultured with different concentrations (0.01 and 10 µg/mL) of CNPs for 3 days and then seeded on coverslips and grown to 80% confluence. The cells were washed once with phosphate-buffered saline (PBS), and 4% paraformaldehyde was used to fix the cells for 10 minutes at room temperature. The cells were solubilized in 0.5% Triton-X100 in PBS for 15 minutes. Then, the cells were incubated in 5% bovine serum albumin for 30 minutes at room temperature followed by an overnight incubation with primary anti-DHX15 (Abcam), anti-p38 (CST), or anti-p65 (CST) antibodies at 4°C. The secondary antibodies that were used were Alexa Fluor 647 goat-anti rabbit IgG, which was applied at a 1:100 dilution for 1 hour. 4′,6-Diamidino-2-phenylindole (DAPI) was used to stain the cell nuclei.
Anti dhx15
Manufactured by Abcam
Anti-DHX15 is a lab equipment product that detects the expression of the DHX15 protein. DHX15 is a helicase enzyme involved in the regulation of RNA processing and splicing. This product can be used to measure the levels of DHX15 in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Coralite488 conjugated affinipure goat anti mouse igg h l, by Proteintech (1 mentions)
Anti phospho p70 s6 kinase thr389, by Cell Signaling Technology (1 mentions)
Anti p70 s6 kinase, by Cell Signaling Technology (1 mentions)
Anti phospho 4e bp1 thr37 46, by Cell Signaling Technology (1 mentions)
Anti ulk1, by Cell Signaling Technology (1 mentions)
Anti actb β actin, by Cell Signaling Technology (1 mentions)
Anti 4e bp1, by Cell Signaling Technology (1 mentions)
Chloroquine diphosphate cq, by Selleck Chemicals (1 mentions)
Anti phospho ulk1, by Cell Signaling Technology (1 mentions)
Anti ki67, by Cell Signaling Technology (1 mentions)
Anti lc3, by Merck Group (1 mentions)
2 protocols using anti dhx15
1
Cellular Response to Nanoparticle Exposure
2
Autophagy Regulation by DHX15 Modulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Baf-A1 (s1413), Torin 1 (s2827), 3-MA (s2767), and Chloroquine diphosphate(CQ, s4157)were purchased from Selleck. The following antibodies were used: anti-LC3 (Sigma, L7645), anti-ACTB/β-actin (Cell Signaling, A5316), anti-phospho-p70 S6 kinase (Thr389) (Cell Signaling, 9234), anti-phospho-4E-BP1 (Thr37/46) (Cell Signaling, 2855), anti-SQSTM1/p62 (MBL PM045), anti-DHX15 (Abcam, ab70454), anti-Ki67 (Cell Signaling, 9449), anti-ULK1 (Cell Signaling, 8054), anti-phospho-ULK1(Ser757) (Cell Signaling, 14202), anti -p70 S6 kinase (Cell Signaling, 2708), anti-4E-BP1 (Cell Signaling, 9452) and CoraLite488–conjugated Affinipure goat anti-mouse IgG (H+L) (Proteintech, SA00013-1). DHX15-Flag was created in the CV702 by standard subcloning. DHX15 plasmid and its negative control were purchased from Genechem (Shanghai, China).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!