Avance 3 600 mhz ft nmr spectrometer

The unknown compound of 10 mg was dissolved in DMSO‑d₆ for NMR spectroscopic analysis. Proton NMR (¹H NMR), carbon-13 NMR (¹³C NMR), correlation spectroscopy (COSY), heteronuclear multiple bond correlation (HMBC), and heteronuclear multiple quantum correlation (HSQC) spectra were recorded on a Bruker AVANCE III 600 MHz FT-NMR spectrometer (Bruker BioSpin, Rheinstetten, Germany) using Me4Si as the internal standard in DMSO‑d₆. Chemical shift (δ) values are in ppm (part per million) and coupling constants (J) are in Hz (hertz).

¹H NMR (600 MHz), ¹³C NMR (150 MHz), and ¹⁵N NMR (61 MHz): Avance III 600
MHz FT-NMR spectrometer (Bruker, Rheinstetten, Germany). ¹H NMR (400 MHz) and ¹³C NMR (100 MHz): Avance 400 FT-NMR
spectrometer (Bruker). ¹H and ¹³C NMR spectra
were referenced to the residual solvent peak [CDCl₃: δ
= 7.26 ppm (¹H), δ = 77.0 ppm (¹³C) or
DMSO-d₆: δ = 2.50 ppm (¹H), δ = 39.5 ppm (¹³C)]. For the ¹⁵N
NMR spectra, nitromethane (δ = 0.0 ppm) was used as an internal
or external standard. In all cases, peak assignments were accomplished
by DEPT135-, HSQC-, and HMBC-NMR experiments. Coupling constants J are given in Hertz (Hz). Multiplicities are described
using the following abbreviations: s = singlet, bs = broad singlet,
d = doublet, dd = doublet of a doublet, ddd = doublet of a doublet
of a doublet, tt = triplet of a triplet, q = quartet, and m = multiplet.
Primary and tertiary carbon atoms have been marked with a “+”,
the secondary with a “–”, and quaternary with
a “C_quat” according to the
peak orientation in the DEPT135 spectra.