CDs were mildly vortexed prior to samples preparation for TEM imaging. Ten microliters of CD sample were drop cast on a 200-mesh copper grid. After waiting for 1 min, the excess fluid was absorbed by lint-free Kimwipes. The transmission electron micrographs were acquired on a JOEL 2100 Cryo TEM machine and imaged by Gatan UltraScan 2k X 2k CCD camera.
Ultrascan 2k x 2k ccd camera
Manufactured by Ametek
The UltraScan 2k x 2k CCD camera is a high-resolution imaging device designed for laboratory applications. It features a 2048 x 2048 pixel sensor, providing a comprehensive field of view and high-quality image capture capabilities.
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Lab products found in correlation
5 protocols using ultrascan 2k x 2k ccd camera
1
TEM Imaging of Colloidal Dispersions
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Ultrastructural Analysis of Newborn Pig Trachea
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Tracheal segments less than 1 mm3 from newborn pigs were cut, rinsed in cold 1X DPBS, and fixed in sodium cacodylate solution with 2.5% glutaraldehyde. Tissues were post-fixed in 2% osmium tetroxide and en bloc stained with 2.5% uranyl acetate. Next, tissues were dehydrated, infiltrated with Eponate 12, and polymerized at 60 °C for one day. 70 nm sections were cut and counterstained with 5% uranyl acetate and Reynold’s lead citrate. Images were taken using a transmission electron microscope (JEOL, JEM1230) with a Gatan UltraScan 2k x 2k CCD camera.
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Ultrastructural Analysis of Newborn Pig Trachea
4
Structural and Magnetic Characterization of Sample Tips
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Structural studies of the tips were carried out by Transmission Electron Microscopy (TEM) using an FEI Titan Cube 60-300 operated at 300 kV, and equipped with a Field Emission Gun (FEG), a Cs corrector for the objective lens and a Gatan Ultrascan 2k x 2k CCD camera. Scanning Transmission Electron Please do not adjust margins Please do not adjust margins Microscopy (STEM) imaging and Electron Energy Loss Spectroscopy (EELS) chemical analyses were performed in a Titan Low Base 60-300 operated at 300 kV fitted with a highbrightness FEG, a Cs corrector for the probe and a Gatan Tridiem 866 ERS spectrometer. STEM-EELS experiments were performed with a probe convergence semi-angle of 25 mrad, an energy dispersion of 0.8 eV, an energy resolution of 1.5 eV, a pixel time of 10-20 ms and an estimated beam current of 250 pA. The local magnetic characterization for quantitative measurements of the magnetic stray field [38] were performed by EH in a Hitachi I2TEM.
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Transmission Electron Microscopy of Biological Samples
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Samples in PBS were diluted 1:10 in water and spotted onto Formvar/carbon 200 mesh copper grids (Ted Pella) that were glow discharge treated (Emitek K100X). The grids were negatively stained with 1% uranyl acetate. Images were acquired with a Thermo Fisher FEI Tecnai 120 keV T12 LaB6 electron microscope equipped with a Gatan Ultrascan 2k x 2k CCD camera and Gatan Digital Micrograph data collection software. Images were processed with Fiji62 (link).
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