NSCLC cells with applied genetic modifications were seeded in 24-well plates at a density of 1.5 × 104 cells per well and were cultured for 96 h. Afterwards, cells were fixed and permeabilized with 1% formaldehyde and 0.2 % Triton X-100, respectively. EdU and DAPI fluorescence dyes were added, and cell nuclei were visualized under the TE200-U fluorescence microscope (NIKON, Tokyo, Japan). EdU-positive nuclei ratio (% vs. DAPI) was recorded.
Te200 u fluorescence microscope
Manufactured by Nikon
Sourced in Japan
The TE200-U is a fluorescence microscope designed for laboratory use. It features a motorized nosepiece with objectives, allowing for quick and precise magnification changes. The microscope is equipped with a high-intensity mercury vapor lamp for fluorescence illumination and a cooled, high-resolution digital camera for capturing fluorescent images. The TE200-U is intended for applications that require fluorescence microscopy techniques.
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Lab products found in correlation
2 protocols using te200 u fluorescence microscope
1
Quantification of Cell Proliferation
2
VSMC Apoptosis Detection Assay
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Transfected VSMCs were fixed and permeabilized with 1% formaldehyde and 0.2% Triton X-100, respectively. After dUTP-end labeling (Clontech, Mountain View, CA, USA) and DAPI staining, VSMCs were visualized by TE200-U fluorescence microscope (Nikon, Tokyo, Japan).
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