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Nucleopore pet membrane

Manufactured by Cytiva
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The Nucleopore PET membrane is a polyethylene terephthalate (PET) membrane designed for use in laboratory applications. It features a uniform pore structure and is characterized by its high porosity and mechanical strength. The Nucleopore PET membrane is a versatile filtration material that can be used in a variety of research and industrial processes.

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Lab products found in correlation

Axioskop 2 plus microscope, by Zeiss (1 mentions) 48 well boyden chamber, by Neuro Probe (1 mentions)

2 protocols using nucleopore pet membrane

1

Boyden Chamber Chemotaxis Assay

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Chemotaxis assays were performed with Boyden chamber methodology as previously described19, 20 using a 48‐well Boyden chamber (Neuroprobe) and Nucleopore PET membrane (Whatman) with 5‐μm‐diameter pores. Cells in a concentration from 0.5 × 106 cells/mL were allowed to migrate for 90 minutes at 37°C and 5% CO2. Only the cells migrated through the pores were counted. In the experiments where pharmacological inhibitors were used, the cells were preincubated with the inhibitors for 30 minutes prior to the chemotaxis/chemokinesis experiments.
Pardali E., Makowski L., Leffers M., Borgscheiper A, & Waltenberger J. (2018). BMP‐2 induces human mononuclear cell chemotaxis and adhesion and modulates monocyte‐to‐macrophage differentiation. Journal of Cellular and Molecular Medicine, 22(11), 5429-5438.
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2

Chemotaxis Assay with Boyden Chamber

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Chemotaxis assays were performed as described previously [7 (link),59 (link)] using a 48-well Boyden chamber (Neuroprobe, Gaithersburg, MD, USA) and Nucleopore PET membrane (Whatman, Maidstone, UK) with 5 µm diameter pores. Cells in a concentration from 0.5 × 106 cells/mL were allowed to migrate for 90 min at 37 °C and 5% CO2. The cells that migrated through the pores were counted. For quantification, migrated cells were counted by 20 high-power fields in four wells using the Axioskop 2 Plus microscope (Carl Zeiss, Jena, Germany).
Semo D., Obergassel J., Dorenkamp M., Hemling P., Strutz J., Hiden U., Müller N., Müller U.A., Zulfikar S.A., Godfrey R, & Waltenberger J. (2023). The Sodium-Glucose Co-Transporter 2 (SGLT2) Inhibitor Empagliflozin Reverses Hyperglycemia-Induced Monocyte and Endothelial Dysfunction Primarily through Glucose Transport-Independent but Redox-Dependent Mechanisms. Journal of Clinical Medicine, 12(4), 1356.
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