Anti bubr1

Manufactured by Fortis Life Sciences

Anti-BubR1 is a laboratory product used for the detection and analysis of BubR1 protein. BubR1 is a key component of the mitotic checkpoint complex, which plays a critical role in ensuring proper chromosome segregation during cell division. The Anti-BubR1 product can be used for various research applications, such as Western blotting, immunoprecipitation, and immunohistochemistry, to study the expression, localization, and function of BubR1 in different cell types and biological systems.

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BubR1 (9 citations)

2 protocols using anti bubr1

Antibody Characterization for PBK Phosphorylation

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The PBK and phospho-PBK (pT9) antibodies were purchased from Cell Signaling Technology (4942 and 4941). Rabbit polyclonal phospho-specific antibodies against PBK T24, S32, and S59 were generated and purified by AbMart. The peptides used for immunizing rabbits were SVLCS-pT-PTINI (T24), INIPA-pS-PFMQK (S32) and RGLSH-pS-PWAVK (S59). The corresponding non-phosphorylated peptides were also synthesized and used for antibody purification and blocking assays. Anti-Flag antibody was from Sigma. Anti-PLK1 was from BioLegend. Anti-β-actin, anti-Mps1/TTK, anti-cyclin E1, and anti-cyclin B antibodies were from Santa Cruz Biotechnology. Anti-aurora-A, anti-CDK2, anti-glutathione S-transferase (GST), anti-BUB1, and anti-BubR1 antibodies were from Bethyl Laboratories. Anti-phospho-S10 H3, anti-YAP, anti-phospho-S127 YAP, anti-phospho-S397 YAP, anti-vimentin, anti-E-cadherin, anti-N-cadherin, anti-CDC25C, anti-CDK4, anti-CDK5, anti-CDK6, anti-Cyclin A2, anti-Cyclin E2, anti-MAD2, anti-phospho-S795 Rb, anti-Wee1, and anti-phospho-S642 Wee1 antibodies were from Cell Signaling Technology. Anti-β-tubulin (Sigma) antibodies were used for immunofluorescence staining.

Stauffer S., Zeng Y., Zhou J., Chen X., Chen Y, & Dong J. (2017). CDK1-mediated Mitotic Phosphorylation of PBK is Involved in Cytokinesis and Inhibits its Oncogenic Activity. Cellular signalling, 39, 74-83.

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Western Blot Antibody Characterization Protocol

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The antibodies and dilutions used for western blots were as follows: rabbit polyclonal anti-FBXW7 (1:500) and anti-AURKA (1:2,000) from Novus; rabbit polyclonal anti-MCL1 (S-19, 1:1,000), anti-Cyclin B1 (1:1,000), anti-phospho-histone H3 (Ser10, 1:500) and mouse monoclonal anti-PTTG1 (1:1,000) from Santa Cruz; mouse monoclonal anti-β-actin (Ac15, 1:20,000) and rabbit polyclonal anti-c-Myc (1:1,000) from Sigma; rabbit polyclonal anti-cleaved caspase-9 (Asp 315, 1:500), anti-cleaved caspase-3 (Asp 175, 1:500) from Cell Signaling; rabbit polyclonal anti-BubR1 (1:3,000) from Bethyl; mouse monoclonal anti-PARP (1:500) from BD Bioscience; mouse monoclonal anti-Cyclin E (1:500) from Monosan; mouse monoclonal anti-PLK1 (1:10,000) from Millipore, and rat monoclonal anti-HA-peroxidase (50 mU/mL) from Roche. For immunohistochemical analyses, anti- FBXW7 (1:2,000), anti- Cyclin E (1:100), anti- MCL1 (1:1,250), anti-AURKA (1:700), and anti-PLK1 (1:1,000) were used.

Gasca J., Flores M.L., Giráldez S., Ruiz-Borrego M., Tortolero M., Romero F., Japón M.A, & Sáez C. (2016). Loss of FBXW7 and accumulation of MCL1 and PLK1 promote paclitaxel resistance in breast cancer. Oncotarget, 7(33), 52751-52765.

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