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Il 17f efluor 660

Manufactured by Thermo Fisher Scientific
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The IL-17F eFluor 660 is a flow cytometry reagent used for the detection and quantification of interleukin-17F (IL-17F) in various cell types. It is a fluorescently-labeled antibody that binds to IL-17F, enabling its analysis through flow cytometry techniques.

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Lab products found in correlation

Cd4 pe, by BD (2 mentions) Cd3 apc cy7, by BD (2 mentions) Cellstripper, by Corning (2 mentions) Cd19 buv395, by BD (2 mentions) Cd11b pe cy7, by BD (2 mentions) Brefeldin a, by Thermo Fisher Scientific (2 mentions) Lsr 2, by FlowJo (2 mentions) Cd8 percp cy5, by BD (2 mentions) Il 17a bv421, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

EFluor 660 (35 citations) IL-17F (28 citations)

2 protocols using il 17f efluor 660

1

Multiparametric Flow Cytometry of Stimulated PBMCs

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Stimulated PBMCs were cultured with Brefeldin A (3µg/ml; eBioscience) added for the last 4hrs. Cells were collected into RPMI/10% FBS with attached cells lifted with Cellstripper (Corning). All reagents were from Biolegend unless otherwise stated. Cells were washed and stained with Zombie Aqua fixable dye, CD19 BUV395 (BD Biosciences), CD4 PE, CD8 PerCP-Cy5.5, CD3 APC-Cy7 and/or CD11b PE-Cy7. Cells were washed, fixed with Fixation Buffer and treated with Permeabilization Buffer, then stained with IL-17F eFluor 660 (eBioscience) and IL-17A BV421 or TNFα APC. Data were acquired on a BD Biosciences LSR II then analyzed with FlowJo 10.0.7. Gating was based on fluorescence minus one and isotype controls. Fig. S1 details gating strategy.
Ip B., Cilfone N., Belkina A.C., DeFuria J., Jagannathan-Bogdan M., Zhu M., Kuchibhatla R., McDonnell M.E., Xiao Q., Kepler T.B., Apovian C.M., Lauffenburger D.A, & Nikolajczyk B.S. (2015). Th17 cytokines differentiate obesity from obesity-associated type 2 diabetes and promote TNFα production. Obesity (Silver Spring, Md.), 24(1), 102-112.
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2

Multiparametric Flow Cytometry of Stimulated PBMCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Stimulated PBMCs were cultured with Brefeldin A (3µg/ml; eBioscience) added for the last 4hrs. Cells were collected into RPMI/10% FBS with attached cells lifted with Cellstripper (Corning). All reagents were from Biolegend unless otherwise stated. Cells were washed and stained with Zombie Aqua fixable dye, CD19 BUV395 (BD Biosciences), CD4 PE, CD8 PerCP-Cy5.5, CD3 APC-Cy7 and/or CD11b PE-Cy7. Cells were washed, fixed with Fixation Buffer and treated with Permeabilization Buffer, then stained with IL-17F eFluor 660 (eBioscience) and IL-17A BV421 or TNFα APC. Data were acquired on a BD Biosciences LSR II then analyzed with FlowJo 10.0.7. Gating was based on fluorescence minus one and isotype controls. Fig. S1 details gating strategy.
Ip B., Cilfone N., Belkina A.C., DeFuria J., Jagannathan-Bogdan M., Zhu M., Kuchibhatla R., McDonnell M.E., Xiao Q., Kepler T.B., Apovian C.M., Lauffenburger D.A, & Nikolajczyk B.S. (2015). Th17 cytokines differentiate obesity from obesity-associated type 2 diabetes and promote TNFα production. Obesity (Silver Spring, Md.), 24(1), 102-112.
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