Antibodies against nrf2

Acetone, 3-mercaptopropionic acid, DSPE and PEG₂₀₀₀ were obtained from RuixiBio (Xi’an, China). DMF and PFOB were purchased from Sigma-Aldrich (St. Louis, USA). Antibodies against Nrf2, collagen Ia1, α-smooth muscle actin (SMA), F4/80, and CD206 were purchased from Cell Signaling Technology (MA, USA). CD86 was purchased from Invitrogen (Carlsbad, CA, USA). Antibodies against GAPDH and HO-1 were purchased from Proteintech (Hubei, China). The HR-conjugated goat anti-rabbit secondary antibody was obtained from Abbkine Scientific (Wuhan, China). Recombinant IL-4 was purchased from BioLegend (CA, USA). The ELISA kits for TGF-β, IL-4, and IL-13 were purchased from Multisciences (Lianke) Biotech (Hangzhou, China), and the test kits for superoxide dismutase (SOD) and malondialdehyde (MDA) were purchased from Beyotime Biotechnology Company (Shanghai, China). DiR was purchased from Bioss (Beijing, China), and DiI and 4',6-diamidino-2-phenylindole (DAPI) were purchased from Beyotime Biotechnology Company (Shanghai, China). Other reagents used in western blot analysis were purchased from Solarbio (Beijing, China).

The concentration of total extracted protein was measured using a BCA kit (Dingguo Bio, China). Western blot analysis was performed using 8%–15% SDS/PAGE gradient gels and transferring onto PVDF membranes (Millipore,MA, USA). Antibodies against Nrf2, NQO-1, HO-1, p- P65, P65, and β-actin (1:1000, all from Cell Signaling Technology, Burlington, MA, USA) were used for detection. Immunoreactive bands were developed using Super ECL Reagent (HaiGene, China).