Frozen ground material (50 mg) was homogenized in 500 μL of 0.1 mM HCl. Samples were centrifuged at 4°C and 20,000 g for 5 min. The supernatant was transferred to an Ultrafree MC 5000 MC NMWL Filter Unit (Millipore) and centrifuged at 5,000 g and 4°C for 90 min. After filtration, samples were diluted 20 times with deionized water and analyzed using the Dionex ICS-2000 system with a KOH gradient, following manufacturer’s protocol (Dionex).
Ultrafree mc 5000 mc nmwl filter unit
Manufactured by Merck Group
The Ultrafree MC 5000 MC NMWL Filter Unit is a laboratory filtration device designed for sample preparation. It features a low-binding, hydrophilic polyethersulfone (PES) membrane with a nominal molecular weight limit (NMWL) of 5,000 Daltons. The device is used for the concentration and desalting of macromolecules such as proteins, peptides, and nucleic acids.
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Lab products found in correlation
2 protocols using ultrafree mc 5000 mc nmwl filter unit
1
Dionex ICS-2000 Ion Chromatography of Frozen Soil
2
Polar Metabolite and Ion Analysis
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A subfraction of the polar phase containing polar metabolites and inorganic ions was filtered using an Ultrafree MC 5000 MC NMWL filter unit (Millipore). Subsequently, anions and cations were analyzed by high-performance anion- and cation-exchange chromatography with conductivity detection facilitated by a Dionex ICS-3000 system as described in detail in Schmidt et al. (2013 (link)).
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