Anti map1lc3a

Paraffin sections of HiF diet induced NAFLD tissues and cryo-sections of patient fatty liver tissue were processed for immunofluorescence staining. For paraffin embedded samples, antigens were retrieved in Target Retrieval Solution (DAKO North America, Inc., Carpinteria, CA, USA) and antigen of frozen samples were retrieved in methanol/acetone (V/V, 1/1). After these sections were blocked with 1% BSA, they were incubated with Anti-MAP1LC3A (1:50, Abcam, USA) and Anti-Beclin 1 (1:100, Abcam, USA). Secondary antibodies, Fluorescein (FITC) AffiniPure goat anti-mouse IgG (1:50, Jackson ImmunoResearch Lab, INC) and Rhodamine Red™-X (RRX) AffiniPure goat anti-rabbit IgG (1:50, Jackson ImmunoResearch Lab, INC., west Grove, PA, USA). Nuclei were counterstained by DAPI in these immune-stained sections. Immunofluorescence images were captured under confocal laser scanning microscopy (Olympus confocal microscope FV1200).

The CHK1 inhibitor Rabusertib was purchased from MedChemExpress. Sheep polyclonal anti‐BubR1 antibody, rabbit monoclonal anti‐γ‐H2A.X and anti‐MAP1LC3A antibodies were obtained from Abcam. The anti‐α‐tubulin‐FITC antibody and Hoechst 33342 were purchased from Sigma. AlexaFluor 488 goat anti‐rabbit antibody and AlexaFluor 594 goat anti‐rabbit antibody were obtained from Invitrogen. The human anti‐centromere CREST antibody was purchased from Fitzgerald Industries International. Mouse polyclonal anti‐BAX, rabbit polyclonal anti‐γ‐H2A.X antibody and anti‐Rad51 antibody were purchased from Proteintech. Rabbit monoclonal anti‐α‐tubulin antibody and phospho‐Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) were purchased from Cell Signaling Technology. FITC‐conjugated and TRITC‐conjugated goat anti‐rabbit IgG, TRITC‐conjugated goat anti‐mouse IgG and TRITC‐conjugated donkey anti‐human IgG were obtained from Zhongshan Golden Bridge Biotechnology, Co., Ltd. All other chemicals and reagents were from Sigma‐Aldrich Corp.