Smart silencer nc

Rat enteric glial cells (HZ-CRL-2690, ATCC, USA) were cultured in Dulbecco's modified Eagle's medium (DMEM; 11965092, Gibco, USA) containing 10% fetal bovine serum (FBS; F8318, Sigma-Aldrich, USA) and 1% penicillin and streptomycin (15140-122, Gibco, USA). The cells were maintained at 37 °C with 5% CO₂ under saturated humidity. Rat H9c2 cells were used as a positive reference to detect the basal expression of LOC108352929 and Phf11. Smart silencer technology was employed to knock down the expression of lncRNA LOC108352929 to observe its effect on the expression of Phf11, TNF-α, and IL-1β in EGCs. EGCs were categorized into four groups: Smart Silencer-LOC108352929 group (SS), Smart silencer-NC group (NC), transfected reagent control group (mock), and unprocessed cell control group (blank). Two replicate wells were used for each group. Smart silencer-LOC108352929 and Smart silencer-NC were custom-synthesized by RIBOBIO (China). After 48 h of culture, cells were collected for RNA extraction.