Briefly, the sections had been repaired with the antigen repair solution, observed by using 0.5% Triton X-100 permeabilized, and blocked with 5% (w/v) bovine serum albumin (BSA). Then the sections were incubated with primary antibodies at 4°C overnight. Primary antibodies used were as follows: TGF-β1 (ab215715, Abcam, 1:200), MMP9 (ab58803, Abcam, 1:200), Platelet endothelial cell adhesion molecule-1 (CD31) (M1511-8, Huabio, 1:200), Cleaved caspase-3 (ab179517, Abcam, 1:200), NKG2-D (sc-515599, Santa Cruz, 1:200), and nucleotide-binding oligomerization domain containing 2 (NOD2) (sc-56168, Santa, 1:200). The slides were incubated with secondary antibodies for 60 min. Finally, nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (c0060, Solarbio), and the slides were visualized by fluorescence microscopy.
M1511 8
Manufactured by Huabio
The M1511-8 is a compact, multi-channel laboratory instrument designed for analytical and research applications. It features eight high-precision measurement channels and can be used to monitor various parameters and signals with consistent accuracy and reliability.
Automatically generated - may contain errors
Lab products found in correlation
Sc 515599, by Santa Cruz Biotechnology (1 mentions)
4 6 diamidino 2 phenylindole dapi c0060, by Solarbio (1 mentions)
Ab215715, by Abcam (1 mentions)
Ab58803, by Abcam (1 mentions)
Ab179517, by Abcam (1 mentions)
Af7001, by Affinity Biosciences (1 mentions)
Af5103, by Affinity Biosciences (1 mentions)
Bs 0581r, by Bioss Antibodies (1 mentions)
Df6087, by Affinity Biosciences (1 mentions)
Af7014, by Affinity Biosciences (1 mentions)
2 protocols using m1511 8
1
Comprehensive Immunohistochemical Characterization
2
Diabetic Wound Healing with MN Patches
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The established animal models were randomly and equivalently divided into six groups, one of which was a control group, in which the rats received no treatments. The other five groups of diabetic rat wounds were treated with the blank-MN patch, ZIF-MN patch, ZG-MN patch, ZH-MN patch, and ZGH-MN patch, respectively. Everyday, the wounds of the rats were examined, and new MN patches were applied and fixed by their self-adhesive property, eliminating the need for additional auxiliary fixation methods. The diabetic wounds were recorded by taking photos on days 0, 7, 14, and 21. After that, the rats were euthanized, and the wound tissues were collected and fixed in a 4% paraformaldehyde buffer for 24 to 48 hours. The obtained tissue samples were then dehydrated, embedded in paraffin, and sectioned into 5- to 7-μm slices for further histology and immunohistochemistry analysis of CD31 (M15118, HUABIO), CD34 (14486-1-Ap, Proteintech), MMP9 (10375-2-Ap, Proteintech), collagen I (Affinity, AF7001), collagen III (Affinity, DF6087), IL-4 (bs-0581R, Bioss), IL-13 (IPB3854, Baijia), IL-6 (Affinity, DF6087), IL-1β (Affinity, AF5103), and TNF-α (Affinity, AF7014).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!