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Gsh gssg assay kit

Manufactured by Promega
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The GSH/GSSG Assay kit is a laboratory equipment product that measures the levels of glutathione (GSH) and glutathione disulfide (GSSG) in biological samples. This kit provides a colorimetric method for the quantitative determination of these two forms of glutathione.

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Lab products found in correlation

Nadph nadp assay kit, by Promega (2 mentions) Cm h2dcfda, by BD (1 mentions) Bodipy 581 591 c11, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) 4 dimethylaminopyridine, by Merck Group (1 mentions) N n 0 dicyclohexylcarbodiimide, by Merck Group (1 mentions) N butyric acid, by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)

Spelling variants of this product

GSH/GSSG-Glo Assay kit (98 citations) GSH/GSSG-GloTM Assay kit (12 citations) GSH assay kit (4 citations) GSH/GSSG-Glo Glutathione Assay Kit (3 citations) GSH/GSSG-glo luminometric assay kit (1 citations)

4 protocols using gsh gssg assay kit

1

Quantifying Cellular Redox Status

Cited 2 times
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The levels of mitochondria and cellular ROS were determined by flow cytometer using CMH2XRos (#M7513) and CM-H2DCF-DA (#C6827) as fluorescent probes (BD Biosciences, San Jose, CA), respectively, as described in previous publications (10 (link),14 (link)). The intracellular levels of GSH/oxidized glutathione (GSSG) and NADPH/NADP+ were measured with a GSH/GSSG Assay kit (#V6612) and a NADPH/NADP+ Assay kit (#G9081) (Promega, WI) according to the manufacturer’s instructions.
Ju H.Q., Lu Y.X., Chen D.L., Zuo Z.X., Liu Z.X., Wu Q.N., Mo H.Y., Wang Z.X., Wang D.S., Pu H.Y., Zeng Z.L., Li B., Xie D., Huang P., Hung M.C., Chiao P.J, & Xu R.H. (2018). Modulation of Redox Homeostasis by Inhibition of MTHFD2 in Colorectal Cancer: Mechanisms and Therapeutic Implications. JNCI Journal of the National Cancer Institute, 111(6), 584-596.
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2

Quantifying Cellular Redox Homeostasis

Cited 1 time
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Cellular ROS levels were determined by flow cytometry using a CM-H2DCF-DA (#D399) Assay kit (Thermo Fisher Scientific, U.S.A.), as described in previous publications [16 (link)]. Lipid ROS levels were measured by flow cytometry using BODIPY 581/591 C11 (#D3861, Thermo Fisher Scientific, U.S.A.) staining. The intracellular levels of GSH/oxidized glutathione (GSSG) and NADPH/NADP+ were measured using a GSH/GSSG Assay kit (#V6612) and an NADPH/NADP+ Assay kit (#G9081) (Promega, WI) according to the manufacturer’s instructions.
Mo H.Y., Wang R.B., Ma M.Y., Zhang Y., Li X.Y., Wen W.R., Han Y, & Tian T. (2024). MTHFD2-mediated redox homeostasis promotes gastric cancer progression under hypoxic conditions. Redox Report : Communications in Free Radical Research, 29(1), 2345455.
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3

Measuring Cellular Glutathione Levels

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HepG2 cells were plated into white and flat-bottom 96-well plates, and cultured for 4 h at 37 °C. Then we treated cells with DMSO or fumarates, and incubated for another 20 h. For fluorescent GSH assay, we first washed cells with Hanks’ balanced salt solution (Solarbio, H1045-500), then determined the levels of reduced and oxidized GSH by GSH/GSSG Assay kit (Promega, V6611), according to the manufacturer’s protocol. Total relative luminescence units (RLU) are graphed as means ± SD. *denotes P < 0.05, NS denotes no significance. Graphed data represents one of three experimental repeats.
Zhang Y., Zhao S., Fu Y., Yan L., Feng Y., Chen Y., Wu Y., Deng Y., Zhang G., Chen Z., Chen Y, & Liu T. (2020). Computational repositioning of dimethyl fumarate for treating alcoholic liver disease. Cell Death & Disease, 11(8), 641.
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4

Synthesis and Antioxidant Evaluation

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Trans-RSV was purchased from TCI Development Co., Ltd. (Shanghai, China). n-Butyric acid was obtained from ACROS (Morris Plains, NJ, USA). N,N0-dicyclohexylcarbodiimide, EDAC, 4-dimethylaminopyridine, DMAP, H2O2, methylthiazolyldiphenyl-tetrazolium bromide (MTT); 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, 98%), isopropanol (99.9%), and dimethyl sulfoxide (DMSO, 99.9%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Tetrahydrofuran (THF) was supplied by Echo Chemical Co., Ltd. (Miaoli County, Taiwan). Dulbecco’s modified Eagle’s medium (DMEM) was supplied by Invitrogen Life Technologies (Carlsbad, MD, USA), fetal bovine serum was supplied by Gibco-BRL (New York, NY, USA), ROS assay kit was supplied by Abcam (#ab113851; Hercules, CA, USA), GSH/GSSG assay kit was supplied by Promega (V6611; Madison, WI, USA), and the CuZn/Mn Superoxide Dismutase (CuZn-SOD/Mn-SOD) Activity Assay Kit (Hydroxylamine Method) was supplied by Elabscience (E-BC-K022-M; Hercules, CA, USA).
Shih M.K., Tain Y.L., Cheng C.M., Hsu C.N., Chen Y.W., Huang H.T., Chang C.I, & Hou C.Y. (2021). Separation and Identification of Resveratrol Butyrate Ester Complexes and Their Bioactivity in HepG2 Cell Models. International Journal of Molecular Sciences, 22(24), 13539.
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