Di water

Manufactured by Thermo Fisher Scientific

Sourced in United States

Deionized (DI) water is a type of purified water commonly used in various laboratory and industrial applications. The core function of DI water is to provide a source of high-purity water by removing dissolved ions, such as salts and minerals, through a process of ion exchange. This results in a water source that is free of impurities, making it suitable for a wide range of applications where consistent and reliable water quality is crucial.

Automatically generated - may contain errors

Lab products found in correlation

8 protocols using di water

Oxidative Modification of Alginate

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Alg-MA was reacted with sodium periodate (Sigma) to open the uronate residues, which form the alginate backbone, creating an aldehyde group.[26 (link), 33 (link)] One percent (w/v) alginate solutions were prepared by dissolving sodium alginate or Alg-MA in DI water. Different volumes of a 3% (w/v) sodium periodate solution were added to the alginate solutions and reacted in the dark for 24 hours at room temperature to achieve distinctly different degrees of oxidation (DOO). The Alg-MA and sodium periodate solutions were mixed together and stirred for 24 hours in a dark environment at room temperature. The product, Alg-MA-Ox, was purified by dialysis in DI water (MWCO 6 kDa, Thermo-Fisher) for three days and lyophilized. Theoretical DOO values were 10, 30 and 50% modification; experimental values were determined by comparing the ratio of alginate methyl protons (5.5 and 5.0 ppm), to the newly formed methyl protons (5.0 ppm).[26 (link)] In addition, the DOM was reassessed after the oxidation reaction (see methods above).

Charron P., Fenn S.L., Poniz A, & Oldinski R.A. (2016). Mechanical Properties and Failure Analysis of Visible Light Crosslinked Alginate-Based Tissue Sealants. Journal of the mechanical behavior of biomedical materials, 59, 314-321.

+ Open protocol

+ Expand

Reusable 3D-Printed Microfluidic Chip Cleaning

Check if the same lab product or an alternative is used in the 5 most similar protocols

The 3D-printed cleaning chip was designed to enable the reusability of microfluidic chips by implementing a simple cleaning procedure to reduce biofouling and cross-contamination between the samples. The cleaning procedure utilized a syringe pump and the four channels of the cleaning chip (Figure 2c,d). First, the biological sample inlet was used: a representative protein sample, 2 mL of bovine serum albumin (BSA) at a high concentration (20 mg/mL), was pumped through the chip, with the cleaning solution and air inlets sealed, using a syringe pump set to 1 mL/min (Thermo Fisher Scientific, Waltham, MA, USA). Following the BSA, air was pumped from the air inlet and through all three non-air channels individually by sealing the remaining two channels, with 0.5 mL of air for each channel, also set at 1 mL/min. The cleaning agent was then pumped, with the biological sample and air inlets sealed, for a total of 10 mL at 1 mL/min. Pure PBS and DI water were tested as the cleaning agent (Thermo Fisher Scientific, Waltham, MA, USA); PBS was determined to be the superior choice. The cleaning procedure ended with a second round of the air to dry all the channels of the chip.

Lepowsky E., Amin R, & Tasoglu S. (2018). Assessing the Reusability of 3D-Printed Photopolymer Microfluidic Chips for Urine Processing. Micromachines, 9(10), 520.

+ Open protocol

+ Expand

Polyelectrolyte Complex Formation Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

A drief detail of materials used is as follows: PDDA (molecular mass 8,500 Da, Polysciences, Inc.); PSS (70 kDa, Sigma-Aldrich, Inc.); ATP, PVP (360 kDa) and KBr (TCI America); carboxymethyl dextran sodium salt (CMDex20, 20 kDa, and CMDex150, 150kDa) (tdb Labs); poly epsilon L-lysine hydrochloride (3,500 to 4,500 Da) and hyaluronic acid sodium salt (HA15, 8 to 15 kDa, and HA80, 70 to 80 kDa) (Carbosynth); dye conjugated BSA (BSA-CF488A and BSA-CF640R) (Biotium, Inc.); and DI water (18 MΩ cm, Thermo Scientific).

Agrawal A., Douglas J.F., Tirrell M, & Karim A. (2022). Manipulation of coacervate droplets with an electric field. Proceedings of the National Academy of Sciences of the United States of America, 119(32), e2203483119.

+ Open protocol

+ Expand

Biofunctionalized Nanoparticle Synthesis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lead (II) chloride (PbCl₂), 1-octadecene (ODE), cadmium oxide (CdO) and sulfur powder (sublimed) were purchased from Alfa Aesar. Oleylamine, oleic acid, 1-octadecene, poly(maleic anhydride-alt-1-octadecene) (PMH; average molecular weight: 30k-50k), 4-morpholineethanesulfonic acid (MES), 4-(dimethylamino)pyridine (DMAP), poly(acrylic acid) (PAA; average molecular weight: 1800), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), and 2-Amino-2-(hydroxymethyl)-1,3-propanediol (tris-base) were purchased from Sigma-Aldrich. Hexane, toluene, chloroform, and DI water were purchased from Fisher Scientific. Methoxy polyethylene glycol amine (mPEG-NH₂; average molecular weight: 5 kD) was purchased from Laysan-Bio. 8-arm polyethylene glycol amine (8Arm-PEG-NH₂‧HCl; average molecular weight: 40 kDa) was purchased from Advanced Biochemicals. All the chemicals were used without further purification. 50 μm capillaries were bought from VitroCom. Purified anti-mouse/human PNAd antibody (Clone: MECA-79), purified anti-mouse CD169 antibody (Clone: 3D6.112) and DRAQ7 (# 424001) were purchased from BioLegend. Anti-mouse CD3 antibody (Clone: KT3) was bought from Bio X Cell.

Wang F., Ren F., Ma Z., Qu L., Gourgues R., Xu C., Baghdasaryan A., Li J., Zadeh I.E., Los J.W., Fognini A., Qin-Dregely J, & Dai H. (2022). In vivo Non-Invasive Confocal Fluorescence Imaging Beyond 1700 nm Using Superconducting Nanowire Single-Photon Detectors. Nature nanotechnology, 17(6), 653-660.

+ Open protocol

+ Expand

Biofunctionalized Nanoparticle Synthesis

Check if the same lab product or an alternative is used in the 5 most similar protocols

+ Open protocol

+ Expand

Synthesis and Characterization of PHEA-C18 Amphiphilic Polymer

Check if the same lab product or an alternative is used in the 5 most similar protocols

First, 291 mg of polysuccinimide (termed PSI; synthesis details in Supporting Information) was dissolved in DMF (ACS grade) at a concentration of 20–25 mg/mL. Then, 81 or 162 mg of octadecylamine was added to the reaction mixture to form PHEA-C₁₈ with a degree of substitution of octadecyl chains (DS_C18) of 20%. After reaction for at least 12 hours under nitrogen at 70 ^° C, the reaction mixture was cooled to room temperature. Then, 161 μL (for DS_C18 of 20%) of ethanolamine was added dropwise and then reacted for another 24 h. Afterward, a dilute solution of excess ethylenediamine was prepared in dry DMF. Then, the reaction mixture was slowly added to the ethylenediamine solution over several minutes. For this step of the reaction, the molar ratio of ethylenediamine to unreacted PSI rings was at least 5:1. After reacting for 3 hours at room temperature, the reaction mixture was dialyzed for at least 2 days against DI water (MWCO 12,000–14,000, Fisherbrand), frozen, and then lyophilized to form a dry powder (Labconco).

Clay N.E., Whittenberg J.J., Leong J., Kumar V., Chen J., Choi I., Liamas E., Schieferstein J.M., Jeong J.H., Kim D.H., Zhang Z.J., Kenis P.J., Kim I.W, & Kong H. (2017). Chemical and Mechanical Modulation of Polymeric Micelle Assembly. Nanoscale, 9(16), 5194-5204.

+ Open protocol

+ Expand

Characterization of Aged BDNPA/F Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

BDNPA/F standards were
provided by CNS Pantex Plant (Amarillo, Texas). The baseline NP was
obtained from a newly opened drum stored at the Los Alamos National
Laboratory, which passed industry standard qualification tests upon
opening. Though this material is more than 50 years old, it is referred
to as the baseline NP because all aged samples were prepared from
this material. DNPOH was synthesized by David Langlois at the Los
Alamos National Laboratory.^{33 (link)} Reference
standards of fluoride, chloride, nitrite, sulfate, acetate, oxalate,
bromide, nitrate, and phosphate were purchased from SPEX CertiPrep
(Metuchen, NJ). DI water, LC/MS grade water, and organic solvents
were purchased from Fisher Chemical (Fair Lawn, NJ).

Chen K., Edgar A.S., Li Z.H., Marina O.C, & Yang D. (2023). Roles of HNOx and Carboxylic Acids in the Thermal Stability of Nitroplasticizer. ACS Omega, 8(16), 14730-14741.

+ Open protocol

+ Expand

Synthesis of Targeted Gold Nanomaterials

Check if the same lab product or an alternative is used in the 5 most similar protocols

Hydrogen tetrachloroaurate(III) trihydrate (Sigma-Aldrich, ≥99.9% trace metals basis), L-glutathione reduced (GSH, Sigma-Aldrich, ≥98.0%), sodium borohydride (Sigma-Aldrich, ≥96%), 1-(3-dimethylaminopropyl)−3-ethylcarbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS, Thermo Scientific) and 2-Amino-2-(hydroxymethyl)−1,3-propanediol (tris-base) were used as received. DI water and indocyanine green (ICG) were purchased from Fisher Scientific. 4-Aminophenylphosphorylcholine (PC) was purchased from Santa Cruz Biotechnology Inc.

Baghdasaryan A., Wang F., Ren F., Ma Z., Li J., Zhou X., Grigoryan L., Xu C, & Dai H. (2022). Phosphorylcholine-conjugated gold-molecular clusters improve signal for Lymph Node NIR-II fluorescence imaging in preclinical cancer models. Nature Communications, 13, 5613.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!