Circular dichroism

Optical rotation data were measured on a MCP 200 dig-ital polarimeter (Anton Paar, Austria). UV and Circular Dichroism spectra were measured on a chirascan Circular Dichroism (Applied Photophysics, UK). HR-ESI-MS data were performed on a Triple TOFTM 5600 ＋ system (AB SCIEX, USA). NMR spectra were recorded on a Bruker avance III HD 400 MHz digital NMR spectrometer (Bruker Optics, Karlsruhe, Germany) with TMS as an internal standard. Column chromatography (CC) was performed with 200～300 mesh silica gel (Qingdao Marine Chemical Factory, China). TLC was performed on silica gel GF254 (Qingdao Marine Chemical Factory, China), spots were visualized under Ultraviolet transmission reflectometer (Shanghai Jingke Industrial, Shanghai). The preparative HPLC was performed on a Semi-preparative HPLC (QuikSep, Beijing, China) with Kromasil 100-5 C 18 column (10 mm×250 mm, 5 μm, Sweden). Subergorgic acid and 2-deoxy-2β-hydroxysubergorgic acid were used to establish GNPS, and their structures were determined by NMR and MS. 2-Deoxy-2β-hydroxysubergorgic acid was also from gorgonian Leptogorgia rigida. [13] The strains in this work were cultured in eight liquid media, eleven bioconversion and four rice media. All of culture media compositions are available in the Supporting Information (Tables S1～S3).