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Bv605 cd45 clone 30 f11

Manufactured by BioLegend
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Bv605-CD45 (clone 30-F11) is a fluorochrome-conjugated antibody used for the detection and analysis of CD45-expressing cells in flow cytometry applications. CD45 is a pan-leukocyte marker expressed on all hematopoietic cells. This product is manufactured by BioLegend.

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Lab products found in correlation

Zombie violet fixable viability kit, by BioLegend (1 mentions) Percp cy5.5 ly 6g, by BioLegend (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Interleukin 2 (il 2), by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

CD45 (30-F11, (72 citations) CD45 (clone 30-F11, (62 citations) Clone 30-F11 (48 citations) CD45-BV605 (25 citations) CD45-BV605 (30-F11), (5 citations)

2 protocols using bv605 cd45 clone 30 f11

1

Flow Cytometry Immune Cell Profiling

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Reagents used for flow cytometry included Zombie VioletTM fixable viability kit (BioLegend, San Diego, CA) and the following anti-mouse antibodies: CD16/32 (clone 93, Fc blocking, BioLegend), Bv605-CD45 (clone 30-F11, BioLegend), PE. Cy7-CD11b (clone M1/70, BioLegend), APC-CD64 (clone X54-5/7.1, BioLegend), FITC-I-A/I-E (MHCII, clone M5/114.15.2, BioLegend), APC. Cy7-Ly6C (clone HK1.4, BioLegend), Bv711-CD3e (clone 145–2 C11, BioLegend), and Percp/Cy5.5-Ly6G (clone IA8, Biolegend). Immune fluorescence staining was performed using the primary antibody Iba1 (rabbit; Wako Cat# 019-1941, RRID: AB_2313566) and secondary goat anti-rabbit antibody; Alexa Fluor-488, Invitrogen).
El Sayed S., Patik I., Redhu N.S., Glickman J.N., Karagiannis K., El Naenaeey E.S., Elmowalid G.A., Abd El Wahab A.M., Snapper S.B, & Horwitz B.H. (2022). CCR2 promotes monocyte recruitment and intestinal inflammation in mice lacking the interleukin-10 receptor. Scientific Reports, 12, 452.
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2

Ex vivo Stimulation of Lung Cells

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2 x106 lung cells were ex vivo stimulated with HDM (10 ug/ml, Greer Labs, Lenoir, NC) and IL-2 (5ng/ml, PeproTech, Cranbury, NJ, USA) for 4 days and production of IL-5 and IL-13 measured by intracellular flow in gated CD4+Foxp3- T cells using the following anti-mouse antibodies: BV605-CD45 (clone 30-F11, BioLegend), BV711-TCRB (clone H57-597, BioLegend), APC-CD4 (clone GK1.5, BioLegend), PE-IL5 (clone TRFK5, BD bioscience) and PReF610-IL13 (clone eBio13A, Invitrogen).
Sethi G.S., Gracias D, & Croft M. (2022). Contribution of circulatory cells to asthma exacerbations and lung tissue-resident CD4 T cell memory. Frontiers in Immunology, 13, 951361.
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