Studies were conducted in adult male (65) and female (67) Sprague-Dawley rats (Envigo, Somerset, NJ) with initial weights ranging from 356 to 504 g in males and 234 to 320 g in females. Rats were individually housed and maintained on a 12-h light/dark cycle with lights on from 6:00 AM to 6:00 PM in an AAALAC International-accredited housing facility. Rats in studies of food-maintained operant responding (referred to below as “food-restricted” rats) had access to 45 mg food pellets (BioServ Dustless Precision Pellets, Flemington, NJ) during operant behavior sessions, and they were given access to unlimited water. Daily food rations (Teklad standard diet - 19% protein; Envigo) for these rats were provided one hr after behavioral sessions and were titrated to maintain daily body weights within 5% of the running mean for all individual subjects by sex for at least three days prior to chemotherapy treatment and were given daily throughout the entirety of operant experiments (8.5 ± 0.5 g/day daily food rations in males and 6.0 ± 0.5 g/day daily food rations in females). For all other rats (referred to below as “free-fed” rats), food and water were available ad libitum in the home cage. Animal-use protocols were approved by the Virginia Commonwealth University Institutional Animal Care and Use Committee.
Teklad standard diet 19 protein
Manufactured by Inotiv
Teklad standard diet - 19% protein is a laboratory animal diet formulated to provide a balanced nutrition for research purposes. The diet contains 19% protein from a combination of animal and plant-based sources. The core function of this product is to serve as a standard laboratory feed for research animals.
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Lab products found in correlation
2 protocols using teklad standard diet 19 protein
1
Operant Behavior in Sprague-Dawley Rats
2
Sprague-Dawley Rat Housing and Diet
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Adult male Sprague-Dawley rats (Envigo, Somerset, NJ) that weighed 275–300 g upon arrival at the laboratory and were housed individually in an AAALAC International-accredited housing facility maintained on a 12-h light/dark cycle with lights on from 6:00 AM to 6:00 PM. All testing occurred during the light phase. Daily food rations (Teklad standard diet—19% protein; Envigo) were provided immediately after behavioral sessions and were titrated to maintain daily body weights within 5% of the running mean for each subject. In addition, rats had access to 45 mg food pellets (BioServ Dustless Precision Grain Pellets, #F0165, Flemington, NJ) during operant behavior sessions. Rats receiving IV paclitaxel received supplemental food as described below during paclitaxel treatment. Water was available ad libitum in the home cage. Animal-use protocols were approved by the Virginia Commonwealth University Institutional Animal Care and Use Committee and were in accordance with the National Academy of Science’s Guide for the Care and Use of Laboratory Animals (National Research Council, 2011 ).
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