Agrimonia eupatoria L. (AG) plant was obtained (CHÁ HUNOS, Lda., Vila Nova de Gaia, Portugal), stored and used according to the supplier’s recommendations. Normal human dermal fibroblast (NHDF) cells were acquired from ATCC—American Type Culture Collection. Cotton fabric was obtained from James H. Heal & Co. Ltd. (Halifax, UK). Resazurin (7-hydroxy-3H-phenoxazin-3-one 10-oxide) dye, 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO), sodium bromide (NaBr), sodium hypochlorite (NaOCl), chitosan (CS) (low molecular weight), sodium hydroxide (NaOH), hydrochloric acid (HCl), sodium chloride (NaCl), Mueller-Hinton broth (MHB), tween 80, dimethyl sulfoxide (DMSO) anhydrous ≥ 99.9%, trypsin, and 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) were purchased from Sigma-Aldrich (Sintra, Portugal). Glacial acetic acid and ethanol absolute were acquired from Fisher Scientific (Porto Salvo, Portugal). Poly(vinyl alcohol) (PVA) (MW 115.000 g/mol) was purchased from VWR International (Carnaxide, Portugal). Nutrient agar (NA), nutrient broth (NB), and agar for microbiology were bought from Fluka (Sintra, Portugal). Brain Heart Infusion (BHI) broth was obtained from Panreac (Barcelona, Spain). Phosphate-buffered saline (PBS) was purchased from Alfa Aesar (Ward Hill, USA). All solvents were of analytical grade and used as received without further purification.
Dimethyl sulfoxide dmso anhydrous 99
Manufactured by Merck Group
Sourced in Portugal
Dimethyl sulfoxide (DMSO) anhydrous ≥ 99.9% is a clear, colorless, polar aprotic solvent. It has a high boiling point and low viscosity. DMSO is commonly used as a solvent and carrier for various chemical and biological applications.
Automatically generated - may contain errors
Lab products found in correlation
Absolute ethanol, by Thermo Fisher Scientific (1 mentions)
3 4 5 dimethyl 2 thiazolyl 2 5 diphenyl 2h tetrazolium bromide mtt, by Merck Group (1 mentions)
Chitosan, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Trypsin, by Merck Group (1 mentions)
Mueller hinton broth (mhb), by Merck Group (1 mentions)
Tween 80, by Merck Group (1 mentions)
Sodium hypochlorite, by Merck Group (1 mentions)
Resazurin, by Merck Group (1 mentions)
2 2 6 6 tetramethylpiperidine 1 oxyl radical, by Merck Group (1 mentions)
Sodium bromide, by Merck Group (1 mentions)
Brain heart infusion broth, by ITW Reagents (1 mentions)
Glacial acetic acid, by Thermo Fisher Scientific (1 mentions)
Acetone, by Thermo Fisher Scientific (1 mentions)
Mowiol 4 88, by Merck Group (1 mentions)
Proteinase k from tritirachium, by Merck Group (1 mentions)
2 protocols using dimethyl sulfoxide dmso anhydrous 99
1
Agrimonia eupatoria L. Bioactive Evaluation
2
Synthesis of BRP-187 Compound
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BRP-187 (4-(4-Chlorophenyl)-5-[4-(quinoline-2-ylmethoxy)phenyl] isoxazol-3-carboxylic acid) was synthesized based on established procedures (Banoglu et al., 2016 (link)). Acetone (>99%, extra pure) was purchased from Acros Organics. Ethyl acetate (ROTISOLV® ≥99.9%, GC Ultra Grade) was purchased from Carl Roth GmbH. Poly(vinyl alcohol) (PVA) (Mowiol 4–88, Mw 31,000 g mol−1), dimethylsulfoxide (DMSO, anhydrous ≥99.9%), proteinase K from Tritirachium and all other materials were received from Sigma Aldrich unless otherwise stated. Ace-DEX was synthesized based on a previously published protocol (Mn 13,700 g mol−1, Ð = 1.41, DScyclic = 1.17 and DSacyclic = 0.87) (Kauffman et al., 2012 (link)). Purified water was used in all stages of NP preparation, purification, and characterization.
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