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Methocult gf h84435

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MethoCult GF H84435 is a methylcellulose-based medium for the in vitro culture of human hematopoietic progenitor cells. It contains recombinant human growth factors to support the growth and differentiation of various hematopoietic lineages.

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MethoCult (224 citations) MethoCult GF (18 citations)

2 protocols using methocult gf h84435

1

Ex Vivo Differentiation of Hematopoietic Progenitors

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2.5 × 104 BM-MNCs were cultured in methylcellulose medium containing erythropoietin, G(M)-CSF, IL-3 and IL-6 (MethoCult GF H84435, Stemcell technologies, Vancouver, Canada) and 2% fetal bovine serum (Integro B.V., Zaandam, The Netherlands) in Petri dishes for 14 days in 37°C 5% CO2 incubator. After 14 days, erythroid progenitor (BFU-E), common myeloid progenitor (CFU-GEMM), and granulocyte-macrophage progenitor (CFU-GM) cell colonies were counted in duplo by an experienced hematology operator.
Noz M.P., Bekkering S., Groh L., Nielen T.M., Lamfers E.J., Schlitzer A., El Messaoudi S., van Royen N., Huys E.H., Preijers F.W., Smeets E.M., Aarntzen E.H., Zhang B., Li Y., Bremmers M.E., van der Velden W.J., Dolstra H., Joosten L.A., Gomes M.E., Netea M.G, & Riksen N.P. (2020). Reprogramming of bone marrow myeloid progenitor cells in patients with severe coronary artery disease. eLife, 9, e60939.
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2

Hematopoietic Differentiation of Embryoid Bodies

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Cells were harvested from day-16 EBs by mechanical dissociation with gentle pipetting in phosphate buffered saline (PBS) and plated in triplicate at 5 × 104 or 105 cells/mL into MethoCult GF H84435 (StemCell Technologies, Vancouver, BC, Canada). Hematopoietic colonies (CFU-E, BFU-E, CFU-GM, and CFU-GEMM) were then counted at day 14.
Flamant S., Chomel J.C., Desterke C., Féraud O., Gobbo E., Mitjavila-Garcia M.T., Foudi A., Griscelli F., Turhan A.G, & Bennaceur-Griscelli A. (2019). Global MicroRNA Profiling Uncovers miR-206 as a Negative Regulator of Hematopoietic Commitment in Human Pluripotent Stem Cells. International Journal of Molecular Sciences, 20(7), 1737.
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