Chromium chip k

Manufactured by 10x Genomics

The Chromium chip K is a core component of the Chromium platform developed by 10x Genomics. It is designed to enable the analysis of single cells or nuclei for a variety of applications, including gene expression profiling, immune repertoire analysis, and chromatin accessibility studies. The Chromium chip K provides the necessary microfluidic infrastructure to partition and process samples for these analyses.

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Lab products found in correlation

Novaseq, by Illumina (1 mentions) Facsaria fusion, by BD (1 mentions) Novaseq 6000, by Illumina (1 mentions)

Spelling variants of this product

Chromium chip (15 citations) Chromium Next GEM Chip K Single Cell Kit (9 citations) Chromium Next GEM Chip K (7 citations)

2 protocols using chromium chip k

Single-cell RNA-seq with Cellular Barcoding

Cited 1 time

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Single-cell RNA-seq experiments were performed by the Brigham and Women’s Hospital Center for Cellular Profiling. Sorted cells were stained with a distinct barcoded antibody (Cell-Hashing antibody, TotalSeq-C, Biolegend) as previously described.¹⁰² (link) After washing, the stained cells were resuspended in 0.4% BSA in PBS at a concentration of 2,000 cells per μL, then loaded onto a single lane (Chromium chip K, 10X Genomics) followed by encapsulation in a lipid droplet (Single Cell 5′kit V2, 10X Genomics) followed by cDNA and library generation according to the manufacturer’s protocol. 5′ mRNA library was sequenced to an average of 50,000 reads per cell, V(D)J library and protein (hashtags) library sequenced to an average of 15,000 reads per cell, all using Illumina Novaseq.

Wang Y., Cho J.W., Kastrunes G., Buck A., Razimbaud C., Culhane A.C., Sun J., Braun D.A., Choueiri T.K., Wu C.J., Jones K., Nguyen Q.D., Zhu Z., Wei K., Zhu Q., Signoretti S., Freeman G.J., Hemberg M, & Marasco W.A. (2024). Immune-restoring CAR-T cells display antitumor activity and reverse immunosuppressive TME in a humanized ccRCC mouse model. iScience, 27(2), 108879.

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Single-cell transcriptomics of CD154+ cells

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For single cell transcriptomics, CD154⁺ cells were isolated by ARTE and further purified by FACS sorting on a BD FACS ARIA Fusion (BD Bioscience) instrument based on dual expression of CD154 and CD69. Cells were sorted into pre-coated low-bind collection tubes, containing 500 μL sterile filtered 1xPBS + 0.2% AB serum. Cells were centrifuged for 10 min at 400xg. The supernatant was carefully removed leaving 20 μL for loading on a Chromium Chip K (10x Genomics) according to the manufacturer’s instructions for processing with the Chromium Next GEM Single Cell 5′Library and Gel Bead Kit v2. TCR single-cell libraries were subsequently prepared with the Chromium Single Cell V(D)J Enrichment Kit, Human T Cell. Libraries were sequenced on Illumina NovaSeq 6000 machine with 2×100 bp for gene expression, aiming for 50,000 reads per cell for gene expression and 5000 reads per cell for TCR libraries.

, & Myers N. (1996). Environmental services of biodiversity.. Proceedings of the National Academy of Sciences of the United States of America, 93(7), 2764-2769.

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