Human lung cancer cell lines (H1299, 95-D, SPCA-1, A549, SK-MES-1, PC-9, H2170 and Hcc-827), human esophageal cancer cell lines (TE-11 and EC-109), human normal esophageal cell lines (HET-1A and HEEpiC), human acute monocytic leukemia cell line (THP-1), and human breast cancer cell line (MCF-7) were purchased from the Cell Bank of the China Academy of Science (Shanghai, China). THP-1, EC-109 and all the human lung cancer cell lines were grown in RPMI-1640 medium (Life Technologies, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS; Gemini, Woodland, CA, USA), 100 IU/ml penicillin and 100 µg/ml streptomycin. TE-11 and MCF-7 cells were grown in Dulbeccos modified Eagles medium (DMEM) culture medium (Life Technologies) supplemented with 10% FBS, 100 IU/ml penicillin and 100 µg/ml streptomycin. HET-1A and HEEpiC were grown in EpiCM-2 complete medium (ScienCell, Carlsbad, CA, USA) with 100 IU/ml penicillin and 100 µg/ml streptomycin. All of the cells were cultured at 37°C in a humidified incubator with 5% CO2.
Het 1a
Manufactured by ScienCell
Sourced in United States
HET-1A is a human esophageal epithelial cell line. It is a primary cell culture derived from normal human esophageal tissue. HET-1A cells exhibit characteristics of normal esophageal epithelial cells and can be used for research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Mcf 7, by Thermo Fisher Scientific (1 mentions)
Thp 1, by Thermo Fisher Scientific (1 mentions)
Ec109, by Thermo Fisher Scientific (1 mentions)
Te 11, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Heepic, by ScienCell (1 mentions)
Kyse520, by National Collection of Authenticated Cell Cultures (1 mentions)
Kyse510, by National Collection of Authenticated Cell Cultures (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Kyse 150, by Thermo Fisher Scientific (1 mentions)
Kyse30, by Thermo Fisher Scientific (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Endothelial cell medium (ecm), by ScienCell (1 mentions)
3 protocols using het 1a
1
Diverse Cancer Cell Lines Cultivation
2
Cultivation of Esophageal Cell Lines
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A normal immortalized human esophagus epithelial cell line HET-1A was obtained from ScienCell Research Laboratory (Carlsbad, CA, USA). Human ESCC cell lines, KYSE-510 and KYSE-520, were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). ESCC cells were grown in Dulbecco's Modified Essential Medium (DMEM) medium (Hyclone, UT, USA) supplemented with 10% fetal bovine serum (FBS) (Gibico, CA, USA) in a 37 °C incubator with 5% CO2 and 95% humidity. HET-1A cells were cultivated under the same cultural conditions in basal medium along with additives obtained from Lonza/Clonetics Corporation (NJ, USA).
3
Esophageal Squamous Cell Carcinoma Tissue Analysis
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A total of 10 pairs of tissue samples, consisting of carcinoma tissue and paracancerous tissue of ESCC, were obtained from the Pathology Department of Tangshan People’s Hospital. The paracancerous tissues, located more than 5.0 cm away from the tumor, were confirmed as normal controls through H&E stains. All procedures were conducted with the approval of the Hospital Ethics Committee (Approval No.: RMYY-LLKS-2023-097). Additionally, ESCC tissue microarrays (HEsoS160CS01) containing approximately 80 pairs of tissue samples were purchased from Outdo Biotech (Shanghai, China).
The human normal esophageal epithelial cell line Het-1A and ESCC cell lines (KYSE-30, KYSE-150, KYSE-410, TE-1, and Eca-109) were obtained from Meisen Cell (Hangzhou, China). Het-1A cells were cultured in Endothelial Cell Medium (1001 + 0025 + 1052 + 0503, ScienCell, San Diego, CA, USA), while KYSE-30 and KYSE-150 cell lines were cultured in Roswell Park Memorial Institute-1640 Medium (21870076, Gibco, Carlsbad, CA, USA) supplemented with 10% Fetal Bovine Serum (10099-141, Gibco) and 1% Penicillin-Streptomycin (15140-122, Gibco). For passaging or subculturing, parietal cells were digested using 0.25% Trypsin-EDTA (25200-056, Gibco). All cell cultures were incubated in a cell incubator at 37 °C with an atmosphere containing 5% CO2.
The human normal esophageal epithelial cell line Het-1A and ESCC cell lines (KYSE-30, KYSE-150, KYSE-410, TE-1, and Eca-109) were obtained from Meisen Cell (Hangzhou, China). Het-1A cells were cultured in Endothelial Cell Medium (1001 + 0025 + 1052 + 0503, ScienCell, San Diego, CA, USA), while KYSE-30 and KYSE-150 cell lines were cultured in Roswell Park Memorial Institute-1640 Medium (21870076, Gibco, Carlsbad, CA, USA) supplemented with 10% Fetal Bovine Serum (10099-141, Gibco) and 1% Penicillin-Streptomycin (15140-122, Gibco). For passaging or subculturing, parietal cells were digested using 0.25% Trypsin-EDTA (25200-056, Gibco). All cell cultures were incubated in a cell incubator at 37 °C with an atmosphere containing 5% CO2.
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