Biotin n hydroxysuccinimide ester biotin nhs

Manufactured by Merck Group

Sourced in United States

The (+)-biotin N-hydroxysuccinimide ester (biotin-NHS) is a biotinylation reagent. It is a derivative of the vitamin biotin that can be used to covalently attach biotin moieties to target molecules, such as proteins, peptides, or other biomolecules, through the reaction of the N-hydroxysuccinimide (NHS) ester with primary amines.

Automatically generated - may contain errors

Lab products found in correlation

Cyanine3 (cy3), by Thermo Fisher Scientific (1 mentions) Dmso solution, by Merck Group (1 mentions) L ascorbic acid, by Merck Group (1 mentions) Whatman, by Cytiva (1 mentions) M13ko7 helper phage, by New England Biolabs (1 mentions) Pcr purification kit, by Qiagen (1 mentions) Plasmid purification kit, by Qiagen (1 mentions) Gel purification kit, by Qiagen (1 mentions) 3 3 5 5 tetramethylbenzidine (tmb), by Merck Group (1 mentions) Mouse anti m13 phage mab horseradish peroxidase hrp, by GE Healthcare (1 mentions) Top10f cells, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

N-hydroxysuccinimide (757 citations) N-hydroxysuccinimide (NHS) (424 citations) NHS-biotin (37 citations) Biotin N-hydroxysuccinimide ester (24 citations)

4 protocols using biotin n hydroxysuccinimide ester biotin nhs

Antibody Conjugation to Microspheres

Check if the same lab product or an alternative is used in the 5 most similar protocols

A mixture of PBS and DMSO solution (SIGMA-ALDRICH, St. Louis, United States) was prepared in a 3:1 ratio. (+)-biotin N-hydroxysuccinimide ester (biotin-NHS; SIGMA-ALDRICH) was added to the mixture to prepare a 1 mg/mL solution. The solution was stirred with the microspheres at room temperature for 4 h to allow biotin to adhere to the surface of the microspheres, and then washed three times with the PBS solution. Subsequently, the reacted microspheres were immersed in a solution of Streptavidin (SAV; Roche, Mannheim, Germany) at a concentration of 50 μg/mL at room temperature for 15 min, and the unreacted SAV was washed with PBS. The biotinylated p75NTR antibody (1 mg/mL) (Bioss, Beijing, China) was then incubated with the microspheres at room temperature for 30 min. After washing three times with PBS, microspheres with surface-modified p75NTR antibody were obtained. The results of antibody conjugation were evaluated using secondary-labeled antibody Cy3 (Invitrogen, Waltham, United States). Observations were conducted under a fluorescence microscope (IX73, Olympus, Japan) to examine the microspheres.

Zou X., Xie B., Peng X., Lu M., Xu D., Yuan H., Zhang Y., Wang D., Zhao M., Liu R, & Wen X. (2024). p75NTR antibody-conjugated microspheres: an approach to guided tissue regeneration by selective recruitment of endogenous periodontal ligament cells. Frontiers in Bioengineering and Biotechnology, 12, 1338029.

+ Open protocol

+ Expand

Synthesis and Characterization of Gold Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tetrachloroauric acid (HAuCl₄•4H₂O, 99.99%), cetyltrimethylammonium bromide (CTAB, 96%), L-ascorbic acid (C₆H₈O₆, 99%), citric acid (C₆H₈O₇), cetyltrimethylammonium chloride (CTAC), hydroxyquinoline (C₉H₇NO, 99%), sodium borohydride (NaBH₄, 99%), silver nitrate (AgNO₃, 99%), p-aminothiophenol (p-ATP), Biotin N-hydroxysuccinimide ester (Biotin-NHS), Phosphate buffered saline (PBS, pH 7.2), and Whatman® qualitative filter paper, Grade 1 (Whatman no. 1) were purchased from Sigma-Aldrich. Invitrogen™ Alexa Fluor® 680 Streptavidin Conjugate was acquired from ThermoFischer Scientific. All chemicals were used without further purification. Ultrapure water (resistivity ~ 18.2 MΩ) was used as solvent throughout the experiments.

Campu A., Susu L., Orzan F., Maniu D., Craciun A.M., Vulpoi A., Roiban L., Focsan M, & Astilean S. (2019). Multimodal Biosensing on Paper-Based Platform Fabricated by Plasmonic Calligraphy Using Gold Nanobypiramids Ink. Frontiers in Chemistry, 7, 55.

+ Open protocol

+ Expand

Preparation and Purification of Recombinant Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Bovine serum albumin (BSA), thyroglobulin, polyethylene glycol 8000 (PEG 8000), Tween 20, isopropyl-β-D-thiogalactopyranoside (IPT), 3, 3’, 5, 5’-tetramethylbenzidine (TMB), and (+)-biotin N-hydroxysuccinimide ester (NHS-biotin) were obtained from Sigma-Aldrich (St. Louis, MO). Helper phage M13KO7 was purchased from New England Biolabs (Ipswich, MA). Mouse anti-M13 phage mAb-horseradish peroxidase (HRP) was purchased from GE Healthcare (Piscataway, NJ). Anti-HA tag antibody-HRP was purchased from Abcam (Cambridge, MA). Chemically competent TOP10F’ cells were obtained from Invitrogen (Carlsbad, CA). The plasmid purification kit, gel purification kit, PCR purification kit, and 6xHis tag purification resins were obtained from Qiagen (Valencia, CA). Electrocompetent ER2738 E. coli cells were purchased from Lucigen Corporation (Middleton, WI). B-PER lysis solution, streptavidin magnetic beads and Zeba Spin desalting columns (MWCO 7k) were purchased from Thermo Pierce Scientific (Rockford, IL). SfiI was purchased from New England Biolabs (Ipswich, MA).
The human sEH [25 (link)], human microsomal epoxide hydrolase (mEH), mouse sEH, rat sEH, rabbit polyclonal anti-human sEH antibody and sEH null mice liver cytosol were prepared in this laboratory. The purified human sEH was boiled for 5 min to obtain the denatured human sEH.

Cui Y., Li D., Morisseau C., Yang J., Wan D., Rossotti M.A., Gee S.J., González-Sapienza G.G, & Hammock B.D. (2015). Heavy Chain Single Domain Antibodies to Detect Native Human Soluble Epoxide Hydrolase. Analytical and bioanalytical chemistry, 407(24), 7275-7283.

+ Open protocol

+ Expand

Biotinylation of Human Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

For the biotinylation of human proteins, 100 µg aliquots of HPG or HK proteins (Enzyme Research Laboratories, South Bend, IN, USA) were incubated for 4 h at 4 °C in 250 µL of 0.1 M NaHCO₃ buffer with 2 µL of biotin N-hydroxysuccinimide ester (NHS-biotin; Sigma) dissolved in dimethylformamide (1 mg/100 µL). The proteins were then dialyzed against PBS buffer, pH 7.4 for 48 h.

Karkowska-Kuleta J., Surowiec M., Gogol M., Koziel J., Potempa B., Potempa J., Kozik A, & Rapala-Kozik M. (2020). Peptidylarginine Deiminase of Porphyromonas gingivalis Modulates the Interactions between Candida albicans Biofilm and Human Plasminogen and High-Molecular-Mass Kininogen. International Journal of Molecular Sciences, 21(7), 2495.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!